Cargando…

Functional diversity of PFKFB3 splice variants in glioblastomas

Tumor cells tend to metabolize glucose through aerobic glycolysis instead of oxidative phosphorylation in mitochondria. One of the rate limiting enzymes of glycolysis is 6-phosphofructo-1-kinase, which is allosterically activated by fructose 2,6-bisphosphate which in turn is produced by 6-phosphofru...

Descripción completa

Detalles Bibliográficos
Autores principales: Heydasch, Ulli, Kessler, Renate, Warnke, Jan-Peter, Eschrich, Klaus, Scholz, Nicole, Bigl, Marina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8263283/
https://www.ncbi.nlm.nih.gov/pubmed/34234350
http://dx.doi.org/10.1371/journal.pone.0241092
_version_ 1783719359110184960
author Heydasch, Ulli
Kessler, Renate
Warnke, Jan-Peter
Eschrich, Klaus
Scholz, Nicole
Bigl, Marina
author_facet Heydasch, Ulli
Kessler, Renate
Warnke, Jan-Peter
Eschrich, Klaus
Scholz, Nicole
Bigl, Marina
author_sort Heydasch, Ulli
collection PubMed
description Tumor cells tend to metabolize glucose through aerobic glycolysis instead of oxidative phosphorylation in mitochondria. One of the rate limiting enzymes of glycolysis is 6-phosphofructo-1-kinase, which is allosterically activated by fructose 2,6-bisphosphate which in turn is produced by 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFK-2/FBPase-2 or PFKFB). Mounting evidence suggests that cancerous tissues overexpress the PFKFB isoenzyme, PFKFB3, being causing enhanced proliferation of cancer cells. Initially, six PFKFB3 splice variants with different C-termini have been documented in humans. More recently, additional splice variants with varying N-termini were discovered the functions of which are to be uncovered. Glioblastoma is one of the deadliest forms of brain tumors. Up to now, the role of PFKFB3 splice variants in the progression and prognosis of glioblastomas is only partially understood. In this study, we first re-categorized the PFKFB3 splice variant repertoire to simplify the denomination. We investigated the impact of increased and decreased levels of PFKFB3-4 (former UBI2K4) and PFKFB3-5 (former variant 5) on the viability and proliferation rate of glioblastoma U87 and HEK-293 cells. The simultaneous knock-down of PFKFB3-4 and PFKFB3-5 led to a decrease in viability and proliferation of U87 and HEK-293 cells as well as a reduction in HEK-293 cell colony formation. Overexpression of PFKFB3-4 but not PFKFB3-5 resulted in increased cell viability and proliferation. This finding contrasts with the common notion that overexpression of PFKFB3 enhances tumor growth, but instead suggests splice variant-specific effects of PFKFB3, apparently with opposing effects on cell behaviour. Strikingly, in line with this result, we found that in human IDH-wildtype glioblastomas, the PFKFB3-4 to PFKFB3-5 ratio was significantly shifted towards PFKFB3-4 when compared to control brain samples. Our findings indicate that the expression level of distinct PFKFB3 splice variants impinges on tumorigenic properties of glioblastomas and that splice pattern may be of important diagnostic value for glioblastoma.
format Online
Article
Text
id pubmed-8263283
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-82632832021-07-19 Functional diversity of PFKFB3 splice variants in glioblastomas Heydasch, Ulli Kessler, Renate Warnke, Jan-Peter Eschrich, Klaus Scholz, Nicole Bigl, Marina PLoS One Research Article Tumor cells tend to metabolize glucose through aerobic glycolysis instead of oxidative phosphorylation in mitochondria. One of the rate limiting enzymes of glycolysis is 6-phosphofructo-1-kinase, which is allosterically activated by fructose 2,6-bisphosphate which in turn is produced by 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFK-2/FBPase-2 or PFKFB). Mounting evidence suggests that cancerous tissues overexpress the PFKFB isoenzyme, PFKFB3, being causing enhanced proliferation of cancer cells. Initially, six PFKFB3 splice variants with different C-termini have been documented in humans. More recently, additional splice variants with varying N-termini were discovered the functions of which are to be uncovered. Glioblastoma is one of the deadliest forms of brain tumors. Up to now, the role of PFKFB3 splice variants in the progression and prognosis of glioblastomas is only partially understood. In this study, we first re-categorized the PFKFB3 splice variant repertoire to simplify the denomination. We investigated the impact of increased and decreased levels of PFKFB3-4 (former UBI2K4) and PFKFB3-5 (former variant 5) on the viability and proliferation rate of glioblastoma U87 and HEK-293 cells. The simultaneous knock-down of PFKFB3-4 and PFKFB3-5 led to a decrease in viability and proliferation of U87 and HEK-293 cells as well as a reduction in HEK-293 cell colony formation. Overexpression of PFKFB3-4 but not PFKFB3-5 resulted in increased cell viability and proliferation. This finding contrasts with the common notion that overexpression of PFKFB3 enhances tumor growth, but instead suggests splice variant-specific effects of PFKFB3, apparently with opposing effects on cell behaviour. Strikingly, in line with this result, we found that in human IDH-wildtype glioblastomas, the PFKFB3-4 to PFKFB3-5 ratio was significantly shifted towards PFKFB3-4 when compared to control brain samples. Our findings indicate that the expression level of distinct PFKFB3 splice variants impinges on tumorigenic properties of glioblastomas and that splice pattern may be of important diagnostic value for glioblastoma. Public Library of Science 2021-07-07 /pmc/articles/PMC8263283/ /pubmed/34234350 http://dx.doi.org/10.1371/journal.pone.0241092 Text en © 2021 Heydasch et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Heydasch, Ulli
Kessler, Renate
Warnke, Jan-Peter
Eschrich, Klaus
Scholz, Nicole
Bigl, Marina
Functional diversity of PFKFB3 splice variants in glioblastomas
title Functional diversity of PFKFB3 splice variants in glioblastomas
title_full Functional diversity of PFKFB3 splice variants in glioblastomas
title_fullStr Functional diversity of PFKFB3 splice variants in glioblastomas
title_full_unstemmed Functional diversity of PFKFB3 splice variants in glioblastomas
title_short Functional diversity of PFKFB3 splice variants in glioblastomas
title_sort functional diversity of pfkfb3 splice variants in glioblastomas
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8263283/
https://www.ncbi.nlm.nih.gov/pubmed/34234350
http://dx.doi.org/10.1371/journal.pone.0241092
work_keys_str_mv AT heydaschulli functionaldiversityofpfkfb3splicevariantsinglioblastomas
AT kesslerrenate functionaldiversityofpfkfb3splicevariantsinglioblastomas
AT warnkejanpeter functionaldiversityofpfkfb3splicevariantsinglioblastomas
AT eschrichklaus functionaldiversityofpfkfb3splicevariantsinglioblastomas
AT scholznicole functionaldiversityofpfkfb3splicevariantsinglioblastomas
AT biglmarina functionaldiversityofpfkfb3splicevariantsinglioblastomas