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Co-immunoprecipitation and semi-quantitative immunoblotting for the analysis of protein-protein interactions
Co-immunoprecipitation (co-IP) of protein complexes from cell lysates is widely used to study protein-protein interactions. However, establishing robust co-IP assays often involves considerable optimization. Moreover, co-IP results are frequently presented in non-quantitative ways. This protocol pre...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8264609/ https://www.ncbi.nlm.nih.gov/pubmed/34278331 http://dx.doi.org/10.1016/j.xpro.2021.100644 |