Optimized protocol for single-molecule RNA FISH to visualize gene expression in S. cerevisiae

Single-molecule RNA fluorescence in situ hybridization (smFISH) allows subcellular visualization, localization, and quantification of endogenous RNA molecules in fixed cells. The spatial and intensity information of each RNA can be used to distinguish mature from nascent transcripts inside each cell...

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Detalles Bibliográficos
Autores principales: Patel, Heta P., Brouwer, Ineke, Lenstra, Tineke L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8264745/
https://www.ncbi.nlm.nih.gov/pubmed/34278333
http://dx.doi.org/10.1016/j.xpro.2021.100647

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8264745/
https://www.ncbi.nlm.nih.gov/pubmed/34278333
http://dx.doi.org/10.1016/j.xpro.2021.100647

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