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Optimized protocol for single-molecule RNA FISH to visualize gene expression in S. cerevisiae
Single-molecule RNA fluorescence in situ hybridization (smFISH) allows subcellular visualization, localization, and quantification of endogenous RNA molecules in fixed cells. The spatial and intensity information of each RNA can be used to distinguish mature from nascent transcripts inside each cell...
Autores principales: | Patel, Heta P., Brouwer, Ineke, Lenstra, Tineke L. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8264745/ https://www.ncbi.nlm.nih.gov/pubmed/34278333 http://dx.doi.org/10.1016/j.xpro.2021.100647 |
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