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Weight changes in hypertensive patients with phlegm-dampness syndrome: an integrated proteomics and metabolomics approach

BACKGROUND: Hypertension (HTN) patients who have phlegm-dampness syndrome (PDS) tend to be obese and have worse outcomes. However, the association of body weight (BW) changes and mechanisms underlying the pathophysiology of HTN-PDS are not well elucidated. This study aims to identify the longitudina...

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Autores principales: Zhang, Chi, Li, Li, Cheng, Shiping, Chowdhury, Debajyoti, Tan, Yong, Liu, Xinru, Zhao, Ning, He, Xiaojuan, Jiang, Miao, Lu, Cheng, Lyu, Aiping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8265088/
https://www.ncbi.nlm.nih.gov/pubmed/34233719
http://dx.doi.org/10.1186/s13020-021-00462-x
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author Zhang, Chi
Li, Li
Cheng, Shiping
Chowdhury, Debajyoti
Tan, Yong
Liu, Xinru
Zhao, Ning
He, Xiaojuan
Jiang, Miao
Lu, Cheng
Lyu, Aiping
author_facet Zhang, Chi
Li, Li
Cheng, Shiping
Chowdhury, Debajyoti
Tan, Yong
Liu, Xinru
Zhao, Ning
He, Xiaojuan
Jiang, Miao
Lu, Cheng
Lyu, Aiping
author_sort Zhang, Chi
collection PubMed
description BACKGROUND: Hypertension (HTN) patients who have phlegm-dampness syndrome (PDS) tend to be obese and have worse outcomes. However, the association of body weight (BW) changes and mechanisms underlying the pathophysiology of HTN-PDS are not well elucidated. This study aims to identify the longitudinal observations associated with the circulating markers discriminating BW changes of individuals with HTN-PDS. METHODS: An integrative approach relying on metabolomics and proteomics was applied to serum samples from HTN-PDS patients in a prospective cohort to identify the plausible mechanistic pathways underpinning HTN-PDS pathophysiology. Study participants were determined to have experienced a weight change if they showed a 5%–15% increase/reduction in BW at the end of the follow-up period. The joint pathway analysis and network analysis were performed using Ingenuity Pathway Analysis (IPA®) on the serum samples obtained from the participants over the period. RESULTS: The study involved 22 HTN-PDS patients who were overweight initially and were able to lose enough weight and 24 HTN-PDS individuals who developed overweight from normal BMI during a one-year follow-up. Our analysis suggested three types of phosphatidylcholine (PC) were altered. PC (22:2(13Z,16Z)/24:1(15Z)) and LysoPC (16:1(9Z)) were decreased in Queryweight gain samples, whereas the levels of PC (14:0/16:0) were increased in weight loss samples. The metabolomic analysis suggested 24 metabolites associated with HTN-PDS. Of them, 13 were up-regulated and 11 were down-regulated. The two-dimensional difference gel electrophoresis (2D DIGE) identified 45 phosphorylated proteins got altered in the HTN-PDS patients, wherein 23 were up-regulated and 22 were down-regulated. Integrated proteomic and metabolomics analyse acknowledged biomarkers PC, Complement C3, C4a/C4b, A2M and SERPINF1 as strong predictors for BW changes in HTN-PDS patients. CONCLUSION: The combined serum proteomic and metabolomic profiling reveals a link between BW change and the complement system activity, altered phosphatidylcholine metabolism in HTN-PDS patients. Future studies with larger cohorts are required to strengthen and validate these findings.
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spelling pubmed-82650882021-07-08 Weight changes in hypertensive patients with phlegm-dampness syndrome: an integrated proteomics and metabolomics approach Zhang, Chi Li, Li Cheng, Shiping Chowdhury, Debajyoti Tan, Yong Liu, Xinru Zhao, Ning He, Xiaojuan Jiang, Miao Lu, Cheng Lyu, Aiping Chin Med Research BACKGROUND: Hypertension (HTN) patients who have phlegm-dampness syndrome (PDS) tend to be obese and have worse outcomes. However, the association of body weight (BW) changes and mechanisms underlying the pathophysiology of HTN-PDS are not well elucidated. This study aims to identify the longitudinal observations associated with the circulating markers discriminating BW changes of individuals with HTN-PDS. METHODS: An integrative approach relying on metabolomics and proteomics was applied to serum samples from HTN-PDS patients in a prospective cohort to identify the plausible mechanistic pathways underpinning HTN-PDS pathophysiology. Study participants were determined to have experienced a weight change if they showed a 5%–15% increase/reduction in BW at the end of the follow-up period. The joint pathway analysis and network analysis were performed using Ingenuity Pathway Analysis (IPA®) on the serum samples obtained from the participants over the period. RESULTS: The study involved 22 HTN-PDS patients who were overweight initially and were able to lose enough weight and 24 HTN-PDS individuals who developed overweight from normal BMI during a one-year follow-up. Our analysis suggested three types of phosphatidylcholine (PC) were altered. PC (22:2(13Z,16Z)/24:1(15Z)) and LysoPC (16:1(9Z)) were decreased in Queryweight gain samples, whereas the levels of PC (14:0/16:0) were increased in weight loss samples. The metabolomic analysis suggested 24 metabolites associated with HTN-PDS. Of them, 13 were up-regulated and 11 were down-regulated. The two-dimensional difference gel electrophoresis (2D DIGE) identified 45 phosphorylated proteins got altered in the HTN-PDS patients, wherein 23 were up-regulated and 22 were down-regulated. Integrated proteomic and metabolomics analyse acknowledged biomarkers PC, Complement C3, C4a/C4b, A2M and SERPINF1 as strong predictors for BW changes in HTN-PDS patients. CONCLUSION: The combined serum proteomic and metabolomic profiling reveals a link between BW change and the complement system activity, altered phosphatidylcholine metabolism in HTN-PDS patients. Future studies with larger cohorts are required to strengthen and validate these findings. BioMed Central 2021-07-07 /pmc/articles/PMC8265088/ /pubmed/34233719 http://dx.doi.org/10.1186/s13020-021-00462-x Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Zhang, Chi
Li, Li
Cheng, Shiping
Chowdhury, Debajyoti
Tan, Yong
Liu, Xinru
Zhao, Ning
He, Xiaojuan
Jiang, Miao
Lu, Cheng
Lyu, Aiping
Weight changes in hypertensive patients with phlegm-dampness syndrome: an integrated proteomics and metabolomics approach
title Weight changes in hypertensive patients with phlegm-dampness syndrome: an integrated proteomics and metabolomics approach
title_full Weight changes in hypertensive patients with phlegm-dampness syndrome: an integrated proteomics and metabolomics approach
title_fullStr Weight changes in hypertensive patients with phlegm-dampness syndrome: an integrated proteomics and metabolomics approach
title_full_unstemmed Weight changes in hypertensive patients with phlegm-dampness syndrome: an integrated proteomics and metabolomics approach
title_short Weight changes in hypertensive patients with phlegm-dampness syndrome: an integrated proteomics and metabolomics approach
title_sort weight changes in hypertensive patients with phlegm-dampness syndrome: an integrated proteomics and metabolomics approach
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8265088/
https://www.ncbi.nlm.nih.gov/pubmed/34233719
http://dx.doi.org/10.1186/s13020-021-00462-x
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