Engineering of the genome editing protein Cas9 to slide along DNA

The genome editing protein Cas9 faces engineering challenges in improving off–target DNA cleavage and low editing efficiency. In this study, we aimed to engineer Cas9 to be able to slide along DNA, which might facilitate genome editing and reduce off-target cleavage. We used two approaches to achiev...

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Detalles Bibliográficos
Autores principales: Banerjee, Trishit, Takahashi, Hiroto, Subekti, Dwiky Rendra Graha, Kamagata, Kiyoto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8266852/
https://www.ncbi.nlm.nih.gov/pubmed/34239016
http://dx.doi.org/10.1038/s41598-021-93685-9

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8266852/
https://www.ncbi.nlm.nih.gov/pubmed/34239016
http://dx.doi.org/10.1038/s41598-021-93685-9

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