Cargando…
Hsp70 Interacts with the TREM-1 Receptor Expressed on Monocytes and Thereby Stimulates Generation of Cytotoxic Lymphocytes Active against MHC-Negative Tumor Cells
The search for and analysis of new ligands for innate immunity receptors are of special significance for understanding the regulatory mechanisms of immune response. Here we show that the major heat shock protein 70 (Hsp70) can bind to and activate TREM-1, the innate immunity receptor expressed on mo...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8267615/ https://www.ncbi.nlm.nih.gov/pubmed/34206968 http://dx.doi.org/10.3390/ijms22136889 |
_version_ | 1783720179243417600 |
---|---|
author | Sharapova, Tatiana N. Romanova, Elena A. Ivanova, Olga K. Yashin, Denis V. Sashchenko, Lidia P. |
author_facet | Sharapova, Tatiana N. Romanova, Elena A. Ivanova, Olga K. Yashin, Denis V. Sashchenko, Lidia P. |
author_sort | Sharapova, Tatiana N. |
collection | PubMed |
description | The search for and analysis of new ligands for innate immunity receptors are of special significance for understanding the regulatory mechanisms of immune response. Here we show that the major heat shock protein 70 (Hsp70) can bind to and activate TREM-1, the innate immunity receptor expressed on monocytes. The Hsp70–TREM-1 interaction activates expression of TNFα and IFNγ mRNAs in monocytes and stimulates IL-2 secretion by PBMCs. Moreover, incubation of PBMCs with Hsp70 leads to an appearance of cytotoxic lymphocyte subpopulations active against the MHC-negative tumor cells. In addition, both the CD4+ T-lymphocytes and CD14+ monocytes are necessary for the Hsp70 signal transduction and a consequent activation of the cytotoxic lymphocytes. We believe that data presented in this study will broaden the views on the involvement of Hsp70 in the antitumor immunity. |
format | Online Article Text |
id | pubmed-8267615 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-82676152021-07-10 Hsp70 Interacts with the TREM-1 Receptor Expressed on Monocytes and Thereby Stimulates Generation of Cytotoxic Lymphocytes Active against MHC-Negative Tumor Cells Sharapova, Tatiana N. Romanova, Elena A. Ivanova, Olga K. Yashin, Denis V. Sashchenko, Lidia P. Int J Mol Sci Article The search for and analysis of new ligands for innate immunity receptors are of special significance for understanding the regulatory mechanisms of immune response. Here we show that the major heat shock protein 70 (Hsp70) can bind to and activate TREM-1, the innate immunity receptor expressed on monocytes. The Hsp70–TREM-1 interaction activates expression of TNFα and IFNγ mRNAs in monocytes and stimulates IL-2 secretion by PBMCs. Moreover, incubation of PBMCs with Hsp70 leads to an appearance of cytotoxic lymphocyte subpopulations active against the MHC-negative tumor cells. In addition, both the CD4+ T-lymphocytes and CD14+ monocytes are necessary for the Hsp70 signal transduction and a consequent activation of the cytotoxic lymphocytes. We believe that data presented in this study will broaden the views on the involvement of Hsp70 in the antitumor immunity. MDPI 2021-06-26 /pmc/articles/PMC8267615/ /pubmed/34206968 http://dx.doi.org/10.3390/ijms22136889 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Sharapova, Tatiana N. Romanova, Elena A. Ivanova, Olga K. Yashin, Denis V. Sashchenko, Lidia P. Hsp70 Interacts with the TREM-1 Receptor Expressed on Monocytes and Thereby Stimulates Generation of Cytotoxic Lymphocytes Active against MHC-Negative Tumor Cells |
title | Hsp70 Interacts with the TREM-1 Receptor Expressed on Monocytes and Thereby Stimulates Generation of Cytotoxic Lymphocytes Active against MHC-Negative Tumor Cells |
title_full | Hsp70 Interacts with the TREM-1 Receptor Expressed on Monocytes and Thereby Stimulates Generation of Cytotoxic Lymphocytes Active against MHC-Negative Tumor Cells |
title_fullStr | Hsp70 Interacts with the TREM-1 Receptor Expressed on Monocytes and Thereby Stimulates Generation of Cytotoxic Lymphocytes Active against MHC-Negative Tumor Cells |
title_full_unstemmed | Hsp70 Interacts with the TREM-1 Receptor Expressed on Monocytes and Thereby Stimulates Generation of Cytotoxic Lymphocytes Active against MHC-Negative Tumor Cells |
title_short | Hsp70 Interacts with the TREM-1 Receptor Expressed on Monocytes and Thereby Stimulates Generation of Cytotoxic Lymphocytes Active against MHC-Negative Tumor Cells |
title_sort | hsp70 interacts with the trem-1 receptor expressed on monocytes and thereby stimulates generation of cytotoxic lymphocytes active against mhc-negative tumor cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8267615/ https://www.ncbi.nlm.nih.gov/pubmed/34206968 http://dx.doi.org/10.3390/ijms22136889 |
work_keys_str_mv | AT sharapovatatianan hsp70interactswiththetrem1receptorexpressedonmonocytesandtherebystimulatesgenerationofcytotoxiclymphocytesactiveagainstmhcnegativetumorcells AT romanovaelenaa hsp70interactswiththetrem1receptorexpressedonmonocytesandtherebystimulatesgenerationofcytotoxiclymphocytesactiveagainstmhcnegativetumorcells AT ivanovaolgak hsp70interactswiththetrem1receptorexpressedonmonocytesandtherebystimulatesgenerationofcytotoxiclymphocytesactiveagainstmhcnegativetumorcells AT yashindenisv hsp70interactswiththetrem1receptorexpressedonmonocytesandtherebystimulatesgenerationofcytotoxiclymphocytesactiveagainstmhcnegativetumorcells AT sashchenkolidiap hsp70interactswiththetrem1receptorexpressedonmonocytesandtherebystimulatesgenerationofcytotoxiclymphocytesactiveagainstmhcnegativetumorcells |