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Transcriptomic and Metabolic Profiling of High-Temperature Treated Storage Roots Reveals the Mechanism of Saccharification in Sweetpotato (Ipomoea batatas (L.) Lam.)

The saccharification of sweetpotato storage roots is a common phenomenon in the cooking process, which determines the edible quality of table use sweetpotato. In the present study, two high saccharified sweetpotato cultivars (Y25, Z13) and one low saccharified cultivar (X27) in two growth periods (S...

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Autores principales: Li, Chen, Kou, Meng, Arisha, Mohamed Hamed, Tang, Wei, Ma, Meng, Yan, Hui, Wang, Xin, Wang, Xiaoxiao, Zhang, Yungang, Liu, Yaju, Gao, Runfei, Li, Qiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8267658/
https://www.ncbi.nlm.nih.gov/pubmed/34206151
http://dx.doi.org/10.3390/ijms22136641
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author Li, Chen
Kou, Meng
Arisha, Mohamed Hamed
Tang, Wei
Ma, Meng
Yan, Hui
Wang, Xin
Wang, Xiaoxiao
Zhang, Yungang
Liu, Yaju
Gao, Runfei
Li, Qiang
author_facet Li, Chen
Kou, Meng
Arisha, Mohamed Hamed
Tang, Wei
Ma, Meng
Yan, Hui
Wang, Xin
Wang, Xiaoxiao
Zhang, Yungang
Liu, Yaju
Gao, Runfei
Li, Qiang
author_sort Li, Chen
collection PubMed
description The saccharification of sweetpotato storage roots is a common phenomenon in the cooking process, which determines the edible quality of table use sweetpotato. In the present study, two high saccharified sweetpotato cultivars (Y25, Z13) and one low saccharified cultivar (X27) in two growth periods (S1, S2) were selected as materials to reveal the molecular mechanism of sweetpotato saccharification treated at high temperature by transcriptome sequencing and non-targeted metabolome determination. The results showed that the comprehensive taste score, sweetness, maltose content and starch change of X27 after steaming were significantly lower than those of Y25 and Z13. Through transcriptome sequencing analysis, 1918 and 1520 differentially expressed genes were obtained in the two periods of S1 and S2, respectively. Some saccharification-related transcription factors including MYB families, WRKY families, bHLH families and inhibitors were screened. Metabolic analysis showed that 162 differentially abundant metabolites related to carbohydrate metabolism were significantly enriched in starch and sucrose capitalization pathways. The correlation analysis between transcriptome and metabolome confirmed that the starch and sucrose metabolic pathways were significantly co-annotated, indicating that it is a vitally important metabolic pathway in the process of sweetpotato saccharification. The data obtained in this study can provide valuable resources for follow-up research on sweetpotato saccharification and will provide new insights and theoretical basis for table use sweetpotato breeding in the future.
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spelling pubmed-82676582021-07-10 Transcriptomic and Metabolic Profiling of High-Temperature Treated Storage Roots Reveals the Mechanism of Saccharification in Sweetpotato (Ipomoea batatas (L.) Lam.) Li, Chen Kou, Meng Arisha, Mohamed Hamed Tang, Wei Ma, Meng Yan, Hui Wang, Xin Wang, Xiaoxiao Zhang, Yungang Liu, Yaju Gao, Runfei Li, Qiang Int J Mol Sci Article The saccharification of sweetpotato storage roots is a common phenomenon in the cooking process, which determines the edible quality of table use sweetpotato. In the present study, two high saccharified sweetpotato cultivars (Y25, Z13) and one low saccharified cultivar (X27) in two growth periods (S1, S2) were selected as materials to reveal the molecular mechanism of sweetpotato saccharification treated at high temperature by transcriptome sequencing and non-targeted metabolome determination. The results showed that the comprehensive taste score, sweetness, maltose content and starch change of X27 after steaming were significantly lower than those of Y25 and Z13. Through transcriptome sequencing analysis, 1918 and 1520 differentially expressed genes were obtained in the two periods of S1 and S2, respectively. Some saccharification-related transcription factors including MYB families, WRKY families, bHLH families and inhibitors were screened. Metabolic analysis showed that 162 differentially abundant metabolites related to carbohydrate metabolism were significantly enriched in starch and sucrose capitalization pathways. The correlation analysis between transcriptome and metabolome confirmed that the starch and sucrose metabolic pathways were significantly co-annotated, indicating that it is a vitally important metabolic pathway in the process of sweetpotato saccharification. The data obtained in this study can provide valuable resources for follow-up research on sweetpotato saccharification and will provide new insights and theoretical basis for table use sweetpotato breeding in the future. MDPI 2021-06-22 /pmc/articles/PMC8267658/ /pubmed/34206151 http://dx.doi.org/10.3390/ijms22136641 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Li, Chen
Kou, Meng
Arisha, Mohamed Hamed
Tang, Wei
Ma, Meng
Yan, Hui
Wang, Xin
Wang, Xiaoxiao
Zhang, Yungang
Liu, Yaju
Gao, Runfei
Li, Qiang
Transcriptomic and Metabolic Profiling of High-Temperature Treated Storage Roots Reveals the Mechanism of Saccharification in Sweetpotato (Ipomoea batatas (L.) Lam.)
title Transcriptomic and Metabolic Profiling of High-Temperature Treated Storage Roots Reveals the Mechanism of Saccharification in Sweetpotato (Ipomoea batatas (L.) Lam.)
title_full Transcriptomic and Metabolic Profiling of High-Temperature Treated Storage Roots Reveals the Mechanism of Saccharification in Sweetpotato (Ipomoea batatas (L.) Lam.)
title_fullStr Transcriptomic and Metabolic Profiling of High-Temperature Treated Storage Roots Reveals the Mechanism of Saccharification in Sweetpotato (Ipomoea batatas (L.) Lam.)
title_full_unstemmed Transcriptomic and Metabolic Profiling of High-Temperature Treated Storage Roots Reveals the Mechanism of Saccharification in Sweetpotato (Ipomoea batatas (L.) Lam.)
title_short Transcriptomic and Metabolic Profiling of High-Temperature Treated Storage Roots Reveals the Mechanism of Saccharification in Sweetpotato (Ipomoea batatas (L.) Lam.)
title_sort transcriptomic and metabolic profiling of high-temperature treated storage roots reveals the mechanism of saccharification in sweetpotato (ipomoea batatas (l.) lam.)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8267658/
https://www.ncbi.nlm.nih.gov/pubmed/34206151
http://dx.doi.org/10.3390/ijms22136641
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