Functional Validation of cas9/GuideRNA Constructs for Site-Directed Mutagenesis of Triticale ABA8′OH1 loci

Cas endonuclease-mediated genome editing provides a long-awaited molecular biological approach to the modification of predefined genomic target sequences in living organisms. Although cas9/guide (g)RNA constructs are straightforward to assemble and can be customized to target virtually any site in t...

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Detalles Bibliográficos
Autores principales: Michalski, Krzysztof, Hertig, Christian, Mańkowski, Dariusz R., Kumlehn, Jochen, Zimny, Janusz, Linkiewicz, Anna M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8269138/
https://www.ncbi.nlm.nih.gov/pubmed/34210100
http://dx.doi.org/10.3390/ijms22137038

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8269138/
https://www.ncbi.nlm.nih.gov/pubmed/34210100
http://dx.doi.org/10.3390/ijms22137038

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