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Functional Validation of cas9/GuideRNA Constructs for Site-Directed Mutagenesis of Triticale ABA8′OH1 loci

Cas endonuclease-mediated genome editing provides a long-awaited molecular biological approach to the modification of predefined genomic target sequences in living organisms. Although cas9/guide (g)RNA constructs are straightforward to assemble and can be customized to target virtually any site in t...

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Detalles Bibliográficos
Autores principales:	Michalski, Krzysztof, Hertig, Christian, Mańkowski, Dariusz R., Kumlehn, Jochen, Zimny, Janusz, Linkiewicz, Anna M.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2021
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8269138/ https://www.ncbi.nlm.nih.gov/pubmed/34210100 http://dx.doi.org/10.3390/ijms22137038

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