Evaluating and Improving Small Subunit rRNA PCR Primer Coverage for Bacteria, Archaea, and Eukaryotes Using Metagenomes from Global Ocean Surveys

Small subunit rRNA (SSU rRNA) amplicon sequencing can quantitatively and comprehensively profile natural microbiomes, representing a critically important tool for studying diverse global ecosystems. However, results will only be accurate if PCR primers perfectly match the rRNA of all organisms prese...

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Detalles Bibliográficos
Autores principales: McNichol, Jesse, Berube, Paul M., Biller, Steven J., Fuhrman, Jed A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2021
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8269242/
https://www.ncbi.nlm.nih.gov/pubmed/34060911
http://dx.doi.org/10.1128/mSystems.00565-21

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8269242/
https://www.ncbi.nlm.nih.gov/pubmed/34060911
http://dx.doi.org/10.1128/mSystems.00565-21

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