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Direct RT-PCR amplification of SARS-CoV-2 from clinical samples using a concentrated viral lysis-amplification buffer prepared with IGEPAL-630

The pandemic of 2019 caused by the novel coronavirus (SARS-CoV-2) is still rapidly spreading worldwide. Nucleic acid amplification serves as the gold standard method for confirmation of COVID-19 infection. However, challenges faced for diagnostic laboratories from undeveloped countries includes shor...

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Detalles Bibliográficos
Autores principales: Castellanos-Gonzalez, Alejandro, Shelite, Thomas R., Lloyd, Nicole, Sadiqova, Aygul, Ping, Ren, Williams-Bouyer, Natalie, Melby, Peter C., Travi, Bruno L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8270935/
https://www.ncbi.nlm.nih.gov/pubmed/34244543
http://dx.doi.org/10.1038/s41598-021-93333-2