Protocol for photoactivation of YAP in cancer cell spheroids embedded in collagen gels

This protocol describes the necessary preparations and procedures to photo-activate Yes-associated protein (YAP) with optoYAP in cancer cell spheroids in 3D collagen matrices. We detail steps for immunofluorescent staining of the resulting YAP-activated HeLa spheroids. In addition, we describe handl...

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Detalles Bibliográficos
Autores principales: Illes, Bernhard, Engelke, Hanna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8271177/
https://www.ncbi.nlm.nih.gov/pubmed/34278337
http://dx.doi.org/10.1016/j.xpro.2021.100657

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8271177/
https://www.ncbi.nlm.nih.gov/pubmed/34278337
http://dx.doi.org/10.1016/j.xpro.2021.100657

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