Protocol for photoactivation of YAP in cancer cell spheroids embedded in collagen gels

This protocol describes the necessary preparations and procedures to photo-activate Yes-associated protein (YAP) with optoYAP in cancer cell spheroids in 3D collagen matrices. We detail steps for immunofluorescent staining of the resulting YAP-activated HeLa spheroids. In addition, we describe handl...

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Detalles Bibliográficos
Autores principales: Illes, Bernhard, Engelke, Hanna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8271177/
https://www.ncbi.nlm.nih.gov/pubmed/34278337
http://dx.doi.org/10.1016/j.xpro.2021.100657
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author Illes, Bernhard
Engelke, Hanna
author_facet Illes, Bernhard
Engelke, Hanna
author_sort Illes, Bernhard
collection PubMed
description This protocol describes the necessary preparations and procedures to photo-activate Yes-associated protein (YAP) with optoYAP in cancer cell spheroids in 3D collagen matrices. We detail steps for immunofluorescent staining of the resulting YAP-activated HeLa spheroids. In addition, we describe handling of optoYAP on 2D substrates. While this protocol focuses on the use of optoYAP in 3D HeLa cell culture, it can be modified for other cell types. For complete details on the use and execution of this protocol, please refer to Illes et al. (2021).
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spelling pubmed-82711772021-07-16 Protocol for photoactivation of YAP in cancer cell spheroids embedded in collagen gels Illes, Bernhard Engelke, Hanna STAR Protoc Protocol This protocol describes the necessary preparations and procedures to photo-activate Yes-associated protein (YAP) with optoYAP in cancer cell spheroids in 3D collagen matrices. We detail steps for immunofluorescent staining of the resulting YAP-activated HeLa spheroids. In addition, we describe handling of optoYAP on 2D substrates. While this protocol focuses on the use of optoYAP in 3D HeLa cell culture, it can be modified for other cell types. For complete details on the use and execution of this protocol, please refer to Illes et al. (2021). Elsevier 2021-07-07 /pmc/articles/PMC8271177/ /pubmed/34278337 http://dx.doi.org/10.1016/j.xpro.2021.100657 Text en © 2021 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Illes, Bernhard
Engelke, Hanna
Protocol for photoactivation of YAP in cancer cell spheroids embedded in collagen gels
title Protocol for photoactivation of YAP in cancer cell spheroids embedded in collagen gels
title_full Protocol for photoactivation of YAP in cancer cell spheroids embedded in collagen gels
title_fullStr Protocol for photoactivation of YAP in cancer cell spheroids embedded in collagen gels
title_full_unstemmed Protocol for photoactivation of YAP in cancer cell spheroids embedded in collagen gels
title_short Protocol for photoactivation of YAP in cancer cell spheroids embedded in collagen gels
title_sort protocol for photoactivation of yap in cancer cell spheroids embedded in collagen gels
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8271177/
https://www.ncbi.nlm.nih.gov/pubmed/34278337
http://dx.doi.org/10.1016/j.xpro.2021.100657
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