Studies on the Structure and Properties of Membrane Phospholipase A(1) Inclusion Bodies Formed at Low Growth Temperatures Using GFP Fusion Strategy

The effect of cultivation temperatures (37, 26, and 18 °C) on the conformational quality of Yersinia pseudotuberculosis phospholipase A(1) (PldA) in inclusion bodies (IBs) was studied using green fluorescent protein (GFP) as a folding reporter. GFP was fused to the C-terminus of PldA to form the Pld...

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Detalles Bibliográficos
Autores principales: Bakholdina, Svetlana I., Stenkova, Anna M., Bystritskaya, Evgenia P., Sidorin, Evgeniy V., Kim, Natalya Yu., Menchinskaya, Ekaterina S., Gorpenchenko, Tatiana Yu., Aminin, Dmitry L., Shved, Nikita A., Solov’eva, Tamara F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8271855/
https://www.ncbi.nlm.nih.gov/pubmed/34203222
http://dx.doi.org/10.3390/molecules26133936

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8271855/
https://www.ncbi.nlm.nih.gov/pubmed/34203222
http://dx.doi.org/10.3390/molecules26133936

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