An optimized protocol with a stepwise approach to identify specific nuclear receptor ligands from cultured mammalian cells

Here, we describe an optimized protocol to identify specific nuclear receptor ligands. First, to rule out any compound interference with luciferase activity per se, we describe an in vitro assay assessing potential inhibition or activation of luciferase enzymatic activity. Second, to comply with EMA...

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Detalles Bibliográficos
Autores principales: Berthier, Alexandre, Staels, Bart, Lefebvre, Philippe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8273406/
https://www.ncbi.nlm.nih.gov/pubmed/34286290
http://dx.doi.org/10.1016/j.xpro.2021.100658

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8273406/
https://www.ncbi.nlm.nih.gov/pubmed/34286290
http://dx.doi.org/10.1016/j.xpro.2021.100658

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