Cargando…
An optimized protocol with a stepwise approach to identify specific nuclear receptor ligands from cultured mammalian cells
Here, we describe an optimized protocol to identify specific nuclear receptor ligands. First, to rule out any compound interference with luciferase activity per se, we describe an in vitro assay assessing potential inhibition or activation of luciferase enzymatic activity. Second, to comply with EMA...
Autores principales: | Berthier, Alexandre, Staels, Bart, Lefebvre, Philippe |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8273406/ https://www.ncbi.nlm.nih.gov/pubmed/34286290 http://dx.doi.org/10.1016/j.xpro.2021.100658 |
Ejemplares similares
-
A Stepwise Screening Protocol for Multiple Myeloma
por: Morawska, Marta, et al.
Publicado: (2023) -
Protocol for Site-Specific Photo-Crosslinking Proteomics to Identify Protein-Protein Interactions in Mammalian Cells
por: Chen, Chengjie, et al.
Publicado: (2020) -
Protocol for nuclear export signal characterization of cGAS in mammalian cells
por: Huang, Yu, et al.
Publicado: (2021) -
A proteomics protocol to identify stimulation-induced binding partners dependent on a specific gene in mammalian cells
por: Zhu, Junji, et al.
Publicado: (2021) -
Protocol for using TRIBE to study RNA-protein interactions and nuclear organization in mammalian cells
por: Biswas, Jeetayu, et al.
Publicado: (2021)