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Building predictive signaling models by perturbing yeast cells with time-varying stimulations resulting in distinct signaling responses
This protocol provides a step-by-step approach to perturb single cells with time-varying stimulation profiles, collect distinct signaling responses, and use these to infer a system of ordinary differential equations to capture and predict dynamics of protein-protein regulation in signal transduction...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8273411/ https://www.ncbi.nlm.nih.gov/pubmed/34286292 http://dx.doi.org/10.1016/j.xpro.2021.100660 |