Reproductive development and genetic structure of the mycoheterotrophic orchid Pogoniopsis schenckii Cogn.
BACKGROUND: Pogoniopsis schenckii Cogn. is a mycoheterotrophic orchid that can be used as a model to understand the influence of mycoheterotrophy at different stages of the reproductive cycle. We aimed to verify the presence of endophytic and epiphytic fungi at each stage of the reproductive process...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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BioMed Central
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8276481/ https://www.ncbi.nlm.nih.gov/pubmed/34253186 http://dx.doi.org/10.1186/s12870-021-03118-y |
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author | Alves, Mariana Ferreira Pinheiro, Fabio Nunes, Carlos Eduardo Pereira Prosdocimi, Francisco Sarzi, Deise Schroder Furtado, Carolina Mayer, Juliana Lischka Sampaio |
author_facet | Alves, Mariana Ferreira Pinheiro, Fabio Nunes, Carlos Eduardo Pereira Prosdocimi, Francisco Sarzi, Deise Schroder Furtado, Carolina Mayer, Juliana Lischka Sampaio |
author_sort | Alves, Mariana Ferreira |
collection | PubMed |
description | BACKGROUND: Pogoniopsis schenckii Cogn. is a mycoheterotrophic orchid that can be used as a model to understand the influence of mycoheterotrophy at different stages of the reproductive cycle. We aimed to verify the presence of endophytic and epiphytic fungi at each stage of the reproductive process and investigated how the breeding system may relate to genetic structure and diversity of populations. In this study we performed anatomical and ultrastructural analyses of the reproductive organs, field tests to confirm the breeding system, and molecular analysis to assess genetic diversity and structure of populations. RESULTS: During the development of the pollen grain, embryo sac and embryogenesis, no fungal infestation was observed. The presence of endophytic fungal hyphae was observed just within floral stems and indehiscent fruit. Beyond assuring the presence of fungus that promote seed germination, specific fungi hyphae in the fruit may affect other process, such as fruit ripening. As other mycoheterotrophic orchids, P. schenckii is autogamous, which may explain the low genetic diversity and high genetic structure in populations. CONCLUSIONS: We discuss an interesting interaction: fungal hyphae in the indehiscent fruit. These fungal hyphae seem to play different roles inside fruit tissues, such as acting in the fruit maturation process and increasing the proximity between fungi and plant seeds even before dispersion occurs. As other mycoheterotrophic orchids, P. schenckii is autogamous, which may explain the low genetic diversity and high genetic structure in populations. Altogether, our findings provide important novel information about the mechanisms shaping ecology and evolution of fragmented populations of mycoheterotrophic plant. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12870-021-03118-y. |
format | Online Article Text |
id | pubmed-8276481 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-82764812021-07-14 Reproductive development and genetic structure of the mycoheterotrophic orchid Pogoniopsis schenckii Cogn. Alves, Mariana Ferreira Pinheiro, Fabio Nunes, Carlos Eduardo Pereira Prosdocimi, Francisco Sarzi, Deise Schroder Furtado, Carolina Mayer, Juliana Lischka Sampaio BMC Plant Biol Research BACKGROUND: Pogoniopsis schenckii Cogn. is a mycoheterotrophic orchid that can be used as a model to understand the influence of mycoheterotrophy at different stages of the reproductive cycle. We aimed to verify the presence of endophytic and epiphytic fungi at each stage of the reproductive process and investigated how the breeding system may relate to genetic structure and diversity of populations. In this study we performed anatomical and ultrastructural analyses of the reproductive organs, field tests to confirm the breeding system, and molecular analysis to assess genetic diversity and structure of populations. RESULTS: During the development of the pollen grain, embryo sac and embryogenesis, no fungal infestation was observed. The presence of endophytic fungal hyphae was observed just within floral stems and indehiscent fruit. Beyond assuring the presence of fungus that promote seed germination, specific fungi hyphae in the fruit may affect other process, such as fruit ripening. As other mycoheterotrophic orchids, P. schenckii is autogamous, which may explain the low genetic diversity and high genetic structure in populations. CONCLUSIONS: We discuss an interesting interaction: fungal hyphae in the indehiscent fruit. These fungal hyphae seem to play different roles inside fruit tissues, such as acting in the fruit maturation process and increasing the proximity between fungi and plant seeds even before dispersion occurs. As other mycoheterotrophic orchids, P. schenckii is autogamous, which may explain the low genetic diversity and high genetic structure in populations. Altogether, our findings provide important novel information about the mechanisms shaping ecology and evolution of fragmented populations of mycoheterotrophic plant. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12870-021-03118-y. BioMed Central 2021-07-12 /pmc/articles/PMC8276481/ /pubmed/34253186 http://dx.doi.org/10.1186/s12870-021-03118-y Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Alves, Mariana Ferreira Pinheiro, Fabio Nunes, Carlos Eduardo Pereira Prosdocimi, Francisco Sarzi, Deise Schroder Furtado, Carolina Mayer, Juliana Lischka Sampaio Reproductive development and genetic structure of the mycoheterotrophic orchid Pogoniopsis schenckii Cogn. |
title | Reproductive development and genetic structure of the mycoheterotrophic orchid Pogoniopsis schenckii Cogn. |
title_full | Reproductive development and genetic structure of the mycoheterotrophic orchid Pogoniopsis schenckii Cogn. |
title_fullStr | Reproductive development and genetic structure of the mycoheterotrophic orchid Pogoniopsis schenckii Cogn. |
title_full_unstemmed | Reproductive development and genetic structure of the mycoheterotrophic orchid Pogoniopsis schenckii Cogn. |
title_short | Reproductive development and genetic structure of the mycoheterotrophic orchid Pogoniopsis schenckii Cogn. |
title_sort | reproductive development and genetic structure of the mycoheterotrophic orchid pogoniopsis schenckii cogn. |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8276481/ https://www.ncbi.nlm.nih.gov/pubmed/34253186 http://dx.doi.org/10.1186/s12870-021-03118-y |
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