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Efficient generation of endogenous protein reporters for mouse development

Fluorescent proteins and epitope tags can reveal protein localization in cells and animals, yet the large size of many tags hinders efficient genome targeting. Accordingly, many studies have relied on characterizing overexpressed proteins, which might not recapitulate endogenous protein activities....

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Detalles Bibliográficos
Autores principales: O'Hagan, Daniel, Kruger, Robin E., Gu, Bin, Ralston, Amy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Company of Biologists Ltd 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8276983/
https://www.ncbi.nlm.nih.gov/pubmed/34036333
http://dx.doi.org/10.1242/dev.197418