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Efficient generation of endogenous protein reporters for mouse development
Fluorescent proteins and epitope tags can reveal protein localization in cells and animals, yet the large size of many tags hinders efficient genome targeting. Accordingly, many studies have relied on characterizing overexpressed proteins, which might not recapitulate endogenous protein activities....
Autores principales: | O'Hagan, Daniel, Kruger, Robin E., Gu, Bin, Ralston, Amy |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Company of Biologists Ltd
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8276983/ https://www.ncbi.nlm.nih.gov/pubmed/34036333 http://dx.doi.org/10.1242/dev.197418 |
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