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Folding of cohesin’s coiled coil is important for Scc2/4-induced association with chromosomes

Cohesin’s association with and translocation along chromosomal DNAs depend on an ATP hydrolysis cycle driving the association and subsequent release of DNA. This involves DNA being ‘clamped’ by Scc2 and ATP-dependent engagement of cohesin’s Smc1 and Smc3 head domains. Scc2’s replacement by Pds5 abro...

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Autores principales: Petela, Naomi J, Gonzalez Llamazares, Andres, Dixon, Sarah, Hu, Bin, Lee, Byung-Gil, Metson, Jean, Seo, Heekyo, Ferrer-Harding, Antonio, Voulgaris, Menelaos, Gligoris, Thomas, Collier, James, Oh, Byung-Ha, Löwe, Jan, Nasmyth, Kim A
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8279761/
https://www.ncbi.nlm.nih.gov/pubmed/34259632
http://dx.doi.org/10.7554/eLife.67268
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author Petela, Naomi J
Gonzalez Llamazares, Andres
Dixon, Sarah
Hu, Bin
Lee, Byung-Gil
Metson, Jean
Seo, Heekyo
Ferrer-Harding, Antonio
Voulgaris, Menelaos
Gligoris, Thomas
Collier, James
Oh, Byung-Ha
Löwe, Jan
Nasmyth, Kim A
author_facet Petela, Naomi J
Gonzalez Llamazares, Andres
Dixon, Sarah
Hu, Bin
Lee, Byung-Gil
Metson, Jean
Seo, Heekyo
Ferrer-Harding, Antonio
Voulgaris, Menelaos
Gligoris, Thomas
Collier, James
Oh, Byung-Ha
Löwe, Jan
Nasmyth, Kim A
author_sort Petela, Naomi J
collection PubMed
description Cohesin’s association with and translocation along chromosomal DNAs depend on an ATP hydrolysis cycle driving the association and subsequent release of DNA. This involves DNA being ‘clamped’ by Scc2 and ATP-dependent engagement of cohesin’s Smc1 and Smc3 head domains. Scc2’s replacement by Pds5 abrogates cohesin’s ATPase and has an important role in halting DNA loop extrusion. The ATPase domains of all SMC proteins are separated from their hinge dimerisation domains by 50-nm-long coiled coils, which have been observed to zip up along their entire length and fold around an elbow, thereby greatly shortening the distance between hinges and ATPase heads. Whether folding exists in vivo or has any physiological importance is not known. We present here a cryo-EM structure of the apo form of cohesin that reveals the structure of folded and zipped-up coils in unprecedented detail and shows that Scc2 can associate with Smc1’s ATPase head even when it is fully disengaged from that of Smc3. Using cysteine-specific crosslinking, we show that cohesin’s coiled coils are frequently folded in vivo, including when cohesin holds sister chromatids together. Moreover, we describe a mutation (SMC1D588Y) within Smc1’s hinge that alters how Scc2 and Pds5 interact with Smc1’s hinge and that enables Scc2 to support loading in the absence of its normal partner Scc4. The mutant phenotype of loading without Scc4 is only explicable if loading depends on an association between Scc2/4 and cohesin’s hinge, which in turn requires coiled coil folding.
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spelling pubmed-82797612021-07-15 Folding of cohesin’s coiled coil is important for Scc2/4-induced association with chromosomes Petela, Naomi J Gonzalez Llamazares, Andres Dixon, Sarah Hu, Bin Lee, Byung-Gil Metson, Jean Seo, Heekyo Ferrer-Harding, Antonio Voulgaris, Menelaos Gligoris, Thomas Collier, James Oh, Byung-Ha Löwe, Jan Nasmyth, Kim A eLife Chromosomes and Gene Expression Cohesin’s association with and translocation along chromosomal DNAs depend on an ATP hydrolysis cycle driving the association and subsequent release of DNA. This involves DNA being ‘clamped’ by Scc2 and ATP-dependent engagement of cohesin’s Smc1 and Smc3 head domains. Scc2’s replacement by Pds5 abrogates cohesin’s ATPase and has an important role in halting DNA loop extrusion. The ATPase domains of all SMC proteins are separated from their hinge dimerisation domains by 50-nm-long coiled coils, which have been observed to zip up along their entire length and fold around an elbow, thereby greatly shortening the distance between hinges and ATPase heads. Whether folding exists in vivo or has any physiological importance is not known. We present here a cryo-EM structure of the apo form of cohesin that reveals the structure of folded and zipped-up coils in unprecedented detail and shows that Scc2 can associate with Smc1’s ATPase head even when it is fully disengaged from that of Smc3. Using cysteine-specific crosslinking, we show that cohesin’s coiled coils are frequently folded in vivo, including when cohesin holds sister chromatids together. Moreover, we describe a mutation (SMC1D588Y) within Smc1’s hinge that alters how Scc2 and Pds5 interact with Smc1’s hinge and that enables Scc2 to support loading in the absence of its normal partner Scc4. The mutant phenotype of loading without Scc4 is only explicable if loading depends on an association between Scc2/4 and cohesin’s hinge, which in turn requires coiled coil folding. eLife Sciences Publications, Ltd 2021-07-14 /pmc/articles/PMC8279761/ /pubmed/34259632 http://dx.doi.org/10.7554/eLife.67268 Text en © 2021, Petela et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Chromosomes and Gene Expression
Petela, Naomi J
Gonzalez Llamazares, Andres
Dixon, Sarah
Hu, Bin
Lee, Byung-Gil
Metson, Jean
Seo, Heekyo
Ferrer-Harding, Antonio
Voulgaris, Menelaos
Gligoris, Thomas
Collier, James
Oh, Byung-Ha
Löwe, Jan
Nasmyth, Kim A
Folding of cohesin’s coiled coil is important for Scc2/4-induced association with chromosomes
title Folding of cohesin’s coiled coil is important for Scc2/4-induced association with chromosomes
title_full Folding of cohesin’s coiled coil is important for Scc2/4-induced association with chromosomes
title_fullStr Folding of cohesin’s coiled coil is important for Scc2/4-induced association with chromosomes
title_full_unstemmed Folding of cohesin’s coiled coil is important for Scc2/4-induced association with chromosomes
title_short Folding of cohesin’s coiled coil is important for Scc2/4-induced association with chromosomes
title_sort folding of cohesin’s coiled coil is important for scc2/4-induced association with chromosomes
topic Chromosomes and Gene Expression
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8279761/
https://www.ncbi.nlm.nih.gov/pubmed/34259632
http://dx.doi.org/10.7554/eLife.67268
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