Cargando…

ACE2-lentiviral transduction enables mouse SARS-CoV-2 infection and mapping of receptor interactions

SARS-CoV-2 uses the human ACE2 (hACE2) receptor for cell attachment and entry, with mouse ACE2 (mACE2) unable to support infection. Herein we describe an ACE2-lentivirus system and illustrate its utility for in vitro and in vivo SARS-CoV-2 infection models. Transduction of non-permissive cell lines...

Descripción completa

Detalles Bibliográficos
Autores principales:	Rawle, Daniel J., Le, Thuy T., Dumenil, Troy, Yan, Kexin, Tang, Bing, Nguyen, Wilson, Watterson, Daniel, Modhiran, Naphak, Hobson-Peters, Jody, Bishop, Cameron, Suhrbier, Andreas
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2021
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8282004/ https://www.ncbi.nlm.nih.gov/pubmed/34214142 http://dx.doi.org/10.1371/journal.ppat.1009723

Ejemplares similares

Evolution of ACE2-independent SARS-CoV-2 infection and mouse adaption after passage in cells expressing human and mouse ACE2
por: Yan, Kexin, et al.
Publicado: (2022)

Warmer ambient air temperatures reduce nasal turbinate and brain infection, but increase lung inflammation in the K18-hACE2 mouse model of COVID-19
por: Dumenil, Troy, et al.
Publicado: (2023)

Widespread discrepancy in Nnt genotypes and genetic backgrounds complicates granzyme A and other knockout mouse studies
por: Rawle, Daniel J, et al.
Publicado: (2022)

Mouse models of COVID-19 recapitulate inflammatory pathways rather than gene expression
por: Bishop, Cameron R., et al.
Publicado: (2022)

Monoclonal Antibodies Specific for SARS-CoV-2 Spike Protein Suitable for Multiple Applications for Current Variants of Concern
por: Morgan, Mahali S., et al.
Publicado: (2022)

Simple rapid in vitro screening method for SARS-CoV-2 anti-virals that identifies potential cytomorbidity-associated false positives
por: Yan, Kexin, et al.
Publicado: (2021)

A Yellow Fever Virus 17D Infection and Disease Mouse Model Used to Evaluate a Chimeric Binjari-Yellow Fever Virus Vaccine
por: Yan, Kexin, et al.
Publicado: (2020)

The Chimeric Binjari-Zika Vaccine Provides Long-Term Protection against ZIKA Virus Challenge
por: Hazlewood, Jessamine E., et al.
Publicado: (2022)

Sequencing of Historical Isolates, K-mer Mining and High Serological Cross-Reactivity with Ross River Virus Argue against the Presence of Getah Virus in Australia
por: Rawle, Daniel J., et al.
Publicado: (2020)

An S1-Nanoparticle Vaccine Protects against SARS-CoV-2 Challenge in K18-hACE2 Mice
por: van Oosten, Linda, et al.
Publicado: (2022)

Inactivation of SARS‐CoV‐2 infectivity in platelet concentrates or plasma following treatment with ultraviolet C light or with methylene blue combined with visible light
por: Hobson‐Peters, Jody, et al.
Publicado: (2023)

A Zika Vaccine Generated Using the Chimeric Insect-Specific Binjari Virus Platform Protects against Fetal Brain Infection in Pregnant Mice
por: Hazlewood, Jessamine E., et al.
Publicado: (2020)

A unified route for flavivirus structures uncovers essential pocket factors conserved across pathogenic viruses
por: Hardy, Joshua M., et al.
Publicado: (2021)

Implications of Dengue Virus Maturation on Vaccine Induced Humoral Immunity in Mice
por: Scott, Connor A. P., et al.
Publicado: (2021)

A Nontoxic Transduction Enhancer Enables Highly Efficient Lentiviral Transduction of Primary Murine T Cells and Hematopoietic Stem Cells
por: Delville, Marianne, et al.
Publicado: (2018)

Process for an efficient lentiviral cell transduction
por: Pirona, Anna Chiara, et al.
Publicado: (2020)

Targeting novel LSD1-dependent ACE2 demethylation domains inhibits SARS-CoV-2 replication
por: Tu, Wen Juan, et al.
Publicado: (2021)

The structure of an infectious immature flavivirus redefines viral architecture and maturation
por: Newton, Natalee D., et al.
Publicado: (2021)

Author Correction: Targeting novel LSD1-dependent ACE2 demethylation domains inhibits SARS-CoV-2 replication
por: Tu, Wen Juan, et al.
Publicado: (2021)

Driving DNA transposition by lentiviral protein transduction
por: Cai, Yujia, et al.
Publicado: (2014)

Synthetic Heparan Sulfate Mimetic Pixatimod (PG545) Potently Inhibits SARS-CoV-2 by Disrupting the Spike–ACE2 Interaction
por: Guimond, Scott E., et al.
Publicado: (2022)

Injection site vaccinology of a recombinant vaccinia-based vector reveals diverse innate immune signatures
por: Hazlewood, Jessamine E., et al.
Publicado: (2021)

Pathophysiological Response to SARS-CoV-2 Infection Detected by Infrared Spectroscopy Enables Rapid and Robust Saliva Screening for COVID-19
por: Kazmer, Seth T., et al.
Publicado: (2022)

Rapid Response Subunit Vaccine Design in the Absence of Structural Information
por: Wijesundara, Danushka K., et al.
Publicado: (2020)

Avoiding lentiviral transduction culture induced MSC senescence
por: Pan, Yung-Wei, et al.
Publicado: (2009)

Optimized protocols for γδ T cell expansion and lentiviral transduction
por: Wang, Rui-Ning, et al.
Publicado: (2019)

A versatile reverse genetics platform for SARS-CoV-2 and other positive-strand RNA viruses
por: Amarilla, Alberto A., et al.
Publicado: (2021)

Subversion of Innate Defenses by the Interplay between DENV and Pre-Existing Enhancing Antibodies: TLRs Signaling Collapse
por: Modhiran, Naphak, et al.
Publicado: (2010)

Lentiviral Transduction of CD34(+) Cells Induces Genome-Wide Epigenetic Modifications
por: Yamagata, Yoshiaki, et al.
Publicado: (2012)

Minimal impact of ZAP on lentiviral vector production and transduction efficiency
por: Sertkaya, Helin, et al.
Publicado: (2021)

Identification of a small molecule for enhancing lentiviral transduction of T cells
por: Malach, Paulina, et al.
Publicado: (2023)

Detection and Quantification of SARS-CoV-2 Receptor Binding Domain Neutralization by a Sensitive Competitive ELISA Assay
por: Shalash, Ahmed O., et al.
Publicado: (2021)

SARS‐CoV‐2 triggers complement activation through interactions with heparan sulfate
por: Lo, Martin W, et al.
Publicado: (2022)

Engineering fusogenic molecules to achieve targeted transduction of enveloped lentiviral vectors
por: Lei, Yuning, et al.
Publicado: (2009)

Lentiviral vector transduction of spermatozoa as a tool for the study of early development
por: Chandrashekran, Anil, et al.
Publicado: (2014)

Toward Tightly Tuned Gene Expression Following Lentiviral Vector Transduction
por: Page, Audrey, et al.
Publicado: (2020)

Cellular Proteo-Transcriptomic Changes in the Immediate Early-Phase of Lentiviral Transduction
por: Linkner, Tamás Richárd, et al.
Publicado: (2021)

Lentiviral transduction facilitates RNA interference in the nematode parasite Nippostrongylus brasiliensis
por: Hagen, Jana, et al.
Publicado: (2021)

Skin-patch delivered subunit vaccine induces broadly neutralising antibodies against SARS-CoV-2 variants of concern
por: McMillan, Christopher L.D., et al.
Publicado: (2022)

Dermal Delivery of a SARS-CoV-2 Subunit Vaccine Induces Immunogenicity against Variants of Concern
por: McMillan, Christopher L. D., et al.
Publicado: (2022)

Cannot write session to /tmp/vufind_sessions/sess_ejk70d8pbsherliq36hacf6kug