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FACS-based isolation of fixed mouse neuronal nuclei for ATAC-seq and Hi-C

The organization of chromatin structure plays a crucial role in gene expression, DNA replication, and repair. Chromatin alterations influence gene expression, and modifications could be associated with genomic instability in the cells during aging or diseases. Here, we provide a modified protocol to...

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Detalles Bibliográficos
Autores principales: Eremenko, Ekaterina, Golova, Anastasia, Stein, Daniel, Einav, Monica, Khrameeva, Ekaterina, Toiber, Debra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8283150/
https://www.ncbi.nlm.nih.gov/pubmed/34308377
http://dx.doi.org/10.1016/j.xpro.2021.100643