Measuring and mitigating PCR bias in microbiota datasets

PCR amplification plays an integral role in the measurement of mixed microbial communities via high-throughput DNA sequencing of the 16S ribosomal RNA (rRNA) gene. Yet PCR is also known to introduce multiple forms of bias in 16S rRNA studies. Here we present a paired modeling and experimental approa...

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Detalles Bibliográficos
Autores principales: Silverman, Justin D., Bloom, Rachael J., Jiang, Sharon, Durand, Heather K., Dallow, Eric, Mukherjee, Sayan, David, Lawrence A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8284789/
https://www.ncbi.nlm.nih.gov/pubmed/34228723
http://dx.doi.org/10.1371/journal.pcbi.1009113

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8284789/
https://www.ncbi.nlm.nih.gov/pubmed/34228723
http://dx.doi.org/10.1371/journal.pcbi.1009113

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