Predictive approaches to guide the expression of recombinant vaccine targets in Escherichia coli: a case study presentation utilising Absynth Biologics Ltd. proprietary Clostridium difficile vaccine antigens

Bacterial expression systems remain a widely used host for recombinant protein production. However, overexpression of recombinant target proteins in bacterial systems such as Escherichia coli can result in poor solubility and the formation of insoluble aggregates. As a consequence, numerous strategi...

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Detalles Bibliográficos
Autores principales: Hussain, Hirra, McKenzie, Edward A, Robinson, Andrew M, Gingles, Neill A, Marston, Fiona, Warwicker, Jim, Dickson, Alan J
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2021
Materias:
Methods and Protocols
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8285303/
https://www.ncbi.nlm.nih.gov/pubmed/34180005
http://dx.doi.org/10.1007/s00253-021-11405-9

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8285303/
https://www.ncbi.nlm.nih.gov/pubmed/34180005
http://dx.doi.org/10.1007/s00253-021-11405-9

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