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Circular L-RNA aptamer promotes target recognition and controls gene activity
Rational design of aptamers to incorporate unnatural nucleotides and special chemical moieties can expand their functional complexity and diversity. Spiegelmer (L-RNA aptamer) is a unique class of aptamer that is composed of unnatural L-RNA nucleotides, and so far there are limited L-RNA aptamer can...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8287958/ https://www.ncbi.nlm.nih.gov/pubmed/34233000 http://dx.doi.org/10.1093/nar/gkab593 |
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author | Ji, Danyang Lyu, Kaixin Zhao, Haizhou Kwok, Chun Kit |
author_facet | Ji, Danyang Lyu, Kaixin Zhao, Haizhou Kwok, Chun Kit |
author_sort | Ji, Danyang |
collection | PubMed |
description | Rational design of aptamers to incorporate unnatural nucleotides and special chemical moieties can expand their functional complexity and diversity. Spiegelmer (L-RNA aptamer) is a unique class of aptamer that is composed of unnatural L-RNA nucleotides, and so far there are limited L-RNA aptamer candidates and applications being reported. Moreover, the target binding properties of current L-RNA aptamers require significant improvement. Here, using L-Apt.4-1c as an example, we develop a simple and robust strategy to generate the first circular L-RNA aptamer, cycL-Apt.4-1c, quantitatively, demonstrate substantial enhancement in binding affinity and selectivity toward its target, and notably report novel applications of circular L-RNA aptamer in controlling RNA–protein interaction, and gene activity including telomerase activity and gene expression. Our approach and findings will be applicable to any L-RNA aptamers and open up a new avenue for diverse applications. |
format | Online Article Text |
id | pubmed-8287958 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-82879582021-07-19 Circular L-RNA aptamer promotes target recognition and controls gene activity Ji, Danyang Lyu, Kaixin Zhao, Haizhou Kwok, Chun Kit Nucleic Acids Res Chemical Biology and Nucleic Acid Chemistry Rational design of aptamers to incorporate unnatural nucleotides and special chemical moieties can expand their functional complexity and diversity. Spiegelmer (L-RNA aptamer) is a unique class of aptamer that is composed of unnatural L-RNA nucleotides, and so far there are limited L-RNA aptamer candidates and applications being reported. Moreover, the target binding properties of current L-RNA aptamers require significant improvement. Here, using L-Apt.4-1c as an example, we develop a simple and robust strategy to generate the first circular L-RNA aptamer, cycL-Apt.4-1c, quantitatively, demonstrate substantial enhancement in binding affinity and selectivity toward its target, and notably report novel applications of circular L-RNA aptamer in controlling RNA–protein interaction, and gene activity including telomerase activity and gene expression. Our approach and findings will be applicable to any L-RNA aptamers and open up a new avenue for diverse applications. Oxford University Press 2021-07-07 /pmc/articles/PMC8287958/ /pubmed/34233000 http://dx.doi.org/10.1093/nar/gkab593 Text en © The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research. https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) ), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
spellingShingle | Chemical Biology and Nucleic Acid Chemistry Ji, Danyang Lyu, Kaixin Zhao, Haizhou Kwok, Chun Kit Circular L-RNA aptamer promotes target recognition and controls gene activity |
title | Circular L-RNA aptamer promotes target recognition and controls gene activity |
title_full | Circular L-RNA aptamer promotes target recognition and controls gene activity |
title_fullStr | Circular L-RNA aptamer promotes target recognition and controls gene activity |
title_full_unstemmed | Circular L-RNA aptamer promotes target recognition and controls gene activity |
title_short | Circular L-RNA aptamer promotes target recognition and controls gene activity |
title_sort | circular l-rna aptamer promotes target recognition and controls gene activity |
topic | Chemical Biology and Nucleic Acid Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8287958/ https://www.ncbi.nlm.nih.gov/pubmed/34233000 http://dx.doi.org/10.1093/nar/gkab593 |
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