DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae

Here we determine the complete genomic sequence of the Gram negative, γ-Proteobacterium Vibrio cholerae El Tor N16961 to be 4,033,460 base pairs (bp). The genome consists of two circular chromosomes of 2,961,146 bp and 1,072,314 bp that together encode 3,885 open reading frames. The vast majority of...

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Detalles Bibliográficos
Autores principales: Heidelberg, John F., Eisen, Jonathan A., Nelson, William C., Clayton, Rebecca A., Gwinn, Michelle L., Dodson, Robert J., Haft, Daniel H., Hickey, Erin K., Peterson, Jeremy D., Umayam, Lowell, Gill, Steven R., Nelson, Karen E., Read, Timothy D., Tettelin, Hervé, Richardson, Delwood, Ermolaeva, Maria D., Vamathevan, Jessica, Bass, Steven, Qin, Haiying, Dragoi, Ioana, Sellers, Patrick, McDonald, Lisa, Utterback, Teresa, Fleishmann, Robert D., Nierman, William C., White, Owen, Salzberg, Steven L., Smith, Hamilton O., Colwell, Rita R., Mekalanos, John J., Venter, J. Craig, Fraser, Claire M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2000
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8288016/
https://www.ncbi.nlm.nih.gov/pubmed/10952301
http://dx.doi.org/10.1038/35020000
Descripción
Sumario:Here we determine the complete genomic sequence of the Gram negative, γ-Proteobacterium Vibrio cholerae El Tor N16961 to be 4,033,460 base pairs (bp). The genome consists of two circular chromosomes of 2,961,146 bp and 1,072,314 bp that together encode 3,885 open reading frames. The vast majority of recognizable genes for essential cell functions (such as DNA replication, transcription, translation and cell-wall biosynthesis) and pathogenicity (for example, toxins, surface antigens and adhesins) are located on the large chromosome. In contrast, the small chromosome contains a larger fraction (59%) of hypothetical genes compared with the large chromosome (42%), and also contains many more genes that appear to have origins other than the γ-Proteobacteria. The small chromosome also carries a gene capture system (the integron island) and host ‘addiction’ genes that are typically found on plasmids; thus, the small chromosome may have originally been a megaplasmid that was captured by an ancestral Vibrio species. The V. cholerae genomic sequence provides a starting point for understanding how a free-living, environmental organism emerged to become a significant human bacterial pathogen. SUPPLEMENTARY INFORMATION: The online version of this article (doi:10.1038/35020000) contains supplementary material, which is available to authorized users.