CasRx-mediated RNA targeting prevents choroidal neovascularization in a mouse model of age-related macular degeneration

RNA-targeting CRISPR system Cas13 offers an efficient approach for manipulating RNA transcripts in vitro. In this perspective, we provide a proof-of-concept demonstration that Cas13-mediated Vegfa knockdown in vivo could prevent the development of laser-induced CNV in mouse model of Age-related macu...

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Autores principales: Zhou, Changyang, Hu, Xinde, Tang, Cheng, Liu, Wenjia, Wang, Shaoran, Zhou, Yingsi, Zhao, Qimeng, Bo, Qiyu, Shi, Linyu, Sun, Xiaodong, Zhou, Haibo, Yang, Hui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Perspectives
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8288881/
https://www.ncbi.nlm.nih.gov/pubmed/34692105
http://dx.doi.org/10.1093/nsr/nwaa033
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author Zhou, Changyang
Hu, Xinde
Tang, Cheng
Liu, Wenjia
Wang, Shaoran
Zhou, Yingsi
Zhao, Qimeng
Bo, Qiyu
Shi, Linyu
Sun, Xiaodong
Zhou, Haibo
Yang, Hui
author_facet Zhou, Changyang
Hu, Xinde
Tang, Cheng
Liu, Wenjia
Wang, Shaoran
Zhou, Yingsi
Zhao, Qimeng
Bo, Qiyu
Shi, Linyu
Sun, Xiaodong
Zhou, Haibo
Yang, Hui
author_sort Zhou, Changyang
collection PubMed
description RNA-targeting CRISPR system Cas13 offers an efficient approach for manipulating RNA transcripts in vitro. In this perspective, we provide a proof-of-concept demonstration that Cas13-mediated Vegfa knockdown in vivo could prevent the development of laser-induced CNV in mouse model of Age-related macular degeneration.
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spelling pubmed-82888812021-10-21 CasRx-mediated RNA targeting prevents choroidal neovascularization in a mouse model of age-related macular degeneration Zhou, Changyang Hu, Xinde Tang, Cheng Liu, Wenjia Wang, Shaoran Zhou, Yingsi Zhao, Qimeng Bo, Qiyu Shi, Linyu Sun, Xiaodong Zhou, Haibo Yang, Hui Natl Sci Rev Perspectives RNA-targeting CRISPR system Cas13 offers an efficient approach for manipulating RNA transcripts in vitro. In this perspective, we provide a proof-of-concept demonstration that Cas13-mediated Vegfa knockdown in vivo could prevent the development of laser-induced CNV in mouse model of Age-related macular degeneration. Oxford University Press 2020-05 2020-03-03 /pmc/articles/PMC8288881/ /pubmed/34692105 http://dx.doi.org/10.1093/nsr/nwaa033 Text en © The Author(s) 2020. Published by Oxford University Press on behalf of China Science Publishing & Media Ltd. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Perspectives
Zhou, Changyang
Hu, Xinde
Tang, Cheng
Liu, Wenjia
Wang, Shaoran
Zhou, Yingsi
Zhao, Qimeng
Bo, Qiyu
Shi, Linyu
Sun, Xiaodong
Zhou, Haibo
Yang, Hui
CasRx-mediated RNA targeting prevents choroidal neovascularization in a mouse model of age-related macular degeneration
title CasRx-mediated RNA targeting prevents choroidal neovascularization in a mouse model of age-related macular degeneration
title_full CasRx-mediated RNA targeting prevents choroidal neovascularization in a mouse model of age-related macular degeneration
title_fullStr CasRx-mediated RNA targeting prevents choroidal neovascularization in a mouse model of age-related macular degeneration
title_full_unstemmed CasRx-mediated RNA targeting prevents choroidal neovascularization in a mouse model of age-related macular degeneration
title_short CasRx-mediated RNA targeting prevents choroidal neovascularization in a mouse model of age-related macular degeneration
title_sort casrx-mediated rna targeting prevents choroidal neovascularization in a mouse model of age-related macular degeneration
topic Perspectives
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8288881/
https://www.ncbi.nlm.nih.gov/pubmed/34692105
http://dx.doi.org/10.1093/nsr/nwaa033
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