Targeted base editing in the plastid genome of Arabidopsis thaliana

Bacterial cytidine deaminase fused to the DNA binding domains of transcription activator-like effector nucleases was recently reported to transiently substitute a targeted C to a T in mitochondrial DNA of mammalian cultured cells(1). We applied this system to targeted base editing in the Arabidopsis...

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Autores principales: Nakazato, Issei, Okuno, Miki, Yamamoto, Hiroshi, Tamura, Yoshiko, Itoh, Takehiko, Shikanai, Toshiharu, Takanashi, Hideki, Tsutsumi, Nobuhiro, Arimura, Shin-ichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Letter
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8289735/
https://www.ncbi.nlm.nih.gov/pubmed/34211131
http://dx.doi.org/10.1038/s41477-021-00954-6
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author Nakazato, Issei
Okuno, Miki
Yamamoto, Hiroshi
Tamura, Yoshiko
Itoh, Takehiko
Shikanai, Toshiharu
Takanashi, Hideki
Tsutsumi, Nobuhiro
Arimura, Shin-ichi
author_facet Nakazato, Issei
Okuno, Miki
Yamamoto, Hiroshi
Tamura, Yoshiko
Itoh, Takehiko
Shikanai, Toshiharu
Takanashi, Hideki
Tsutsumi, Nobuhiro
Arimura, Shin-ichi
author_sort Nakazato, Issei
collection PubMed
description Bacterial cytidine deaminase fused to the DNA binding domains of transcription activator-like effector nucleases was recently reported to transiently substitute a targeted C to a T in mitochondrial DNA of mammalian cultured cells(1). We applied this system to targeted base editing in the Arabidopsis thaliana plastid genome. The targeted Cs were homoplasmically substituted to Ts in some plantlets of the T(1) generation and the mutations were inherited by their offspring independently of their nuclear-introduced vectors.
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spelling pubmed-82897352021-07-23 Targeted base editing in the plastid genome of Arabidopsis thaliana Nakazato, Issei Okuno, Miki Yamamoto, Hiroshi Tamura, Yoshiko Itoh, Takehiko Shikanai, Toshiharu Takanashi, Hideki Tsutsumi, Nobuhiro Arimura, Shin-ichi Nat Plants Letter Bacterial cytidine deaminase fused to the DNA binding domains of transcription activator-like effector nucleases was recently reported to transiently substitute a targeted C to a T in mitochondrial DNA of mammalian cultured cells(1). We applied this system to targeted base editing in the Arabidopsis thaliana plastid genome. The targeted Cs were homoplasmically substituted to Ts in some plantlets of the T(1) generation and the mutations were inherited by their offspring independently of their nuclear-introduced vectors. Nature Publishing Group UK 2021-07-01 2021 /pmc/articles/PMC8289735/ /pubmed/34211131 http://dx.doi.org/10.1038/s41477-021-00954-6 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Letter
Nakazato, Issei
Okuno, Miki
Yamamoto, Hiroshi
Tamura, Yoshiko
Itoh, Takehiko
Shikanai, Toshiharu
Takanashi, Hideki
Tsutsumi, Nobuhiro
Arimura, Shin-ichi
Targeted base editing in the plastid genome of Arabidopsis thaliana
title Targeted base editing in the plastid genome of Arabidopsis thaliana
title_full Targeted base editing in the plastid genome of Arabidopsis thaliana
title_fullStr Targeted base editing in the plastid genome of Arabidopsis thaliana
title_full_unstemmed Targeted base editing in the plastid genome of Arabidopsis thaliana
title_short Targeted base editing in the plastid genome of Arabidopsis thaliana
title_sort targeted base editing in the plastid genome of arabidopsis thaliana
topic Letter
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8289735/
https://www.ncbi.nlm.nih.gov/pubmed/34211131
http://dx.doi.org/10.1038/s41477-021-00954-6
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