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Platelet-concentrated and platelet poor-plasma promote different pattern on immunohistochemical expression of TGF-β1, however they impairs the osteoneogensis in calvarial defects treated with autograft due suppression of osteocalcin

PURPOSE: Herein we evaluated the effects of platelet concentrate (PC) and platelet-poor plasma (PPP) on bone repair using noncritical defects in the calvaria of rabbits and compared them to the presence of TGF-β1 and osteocalcin on reparative sites. METHODS: Five noncritical defects of 8.7 mm in dia...

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Autores principales: de Oliveira-Filho, Marco Antonio, Souza, Marcelo, Tabushi, Fernando Issamu, Almeida, Luís Eduardo, Figueiredo, Fernanda Pirajá, Lourenço, Elora Sampaio, Giovanini, Allan Fernando, Malafaia, Osvaldo, Ribas, Jurandir Marcondes
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8291903/
https://www.ncbi.nlm.nih.gov/pubmed/34287607
http://dx.doi.org/10.1590/ACB360604
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author de Oliveira-Filho, Marco Antonio
Souza, Marcelo
Tabushi, Fernando Issamu
Almeida, Luís Eduardo
Figueiredo, Fernanda Pirajá
Lourenço, Elora Sampaio
Giovanini, Allan Fernando
Malafaia, Osvaldo
Ribas, Jurandir Marcondes
author_facet de Oliveira-Filho, Marco Antonio
Souza, Marcelo
Tabushi, Fernando Issamu
Almeida, Luís Eduardo
Figueiredo, Fernanda Pirajá
Lourenço, Elora Sampaio
Giovanini, Allan Fernando
Malafaia, Osvaldo
Ribas, Jurandir Marcondes
author_sort de Oliveira-Filho, Marco Antonio
collection PubMed
description PURPOSE: Herein we evaluated the effects of platelet concentrate (PC) and platelet-poor plasma (PPP) on bone repair using noncritical defects in the calvaria of rabbits and compared them to the presence of TGF-β1 and osteocalcin on reparative sites. METHODS: Five noncritical defects of 8.7 mm in diameter were created on the calvaria of 15 animals. Each defect was treated differently, using autograft (ABG), ABG associated with PC (ABG + PC), ABG with PPP (ABG + PPP), isolated PPP, and blood clot (control). The animals were submitted to euthanasia on the second, fourth and sixth week post-surgery. RESULTS: The defects that received ABG+PC or PPP demonstrated lower bone formation when compared to specimens that received ABG in the same period. These results coincided to significant higher immunopositivity for TGF-β1 for specimens that received PC, and lower presence of cytokine in the group PPP. However, either higher or lower presence of TGF-β1 were also correlated to lower presence of osteocalcin. Likewise, these results were similar to findings in specimens treated only with PPP when compared to control. CONCLUSIONS: PC and PPP were not effective when applied in association with ABG. Similarly, isolated use of PPP was not beneficial in optimizing the bone repair.
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spelling pubmed-82919032021-08-03 Platelet-concentrated and platelet poor-plasma promote different pattern on immunohistochemical expression of TGF-β1, however they impairs the osteoneogensis in calvarial defects treated with autograft due suppression of osteocalcin de Oliveira-Filho, Marco Antonio Souza, Marcelo Tabushi, Fernando Issamu Almeida, Luís Eduardo Figueiredo, Fernanda Pirajá Lourenço, Elora Sampaio Giovanini, Allan Fernando Malafaia, Osvaldo Ribas, Jurandir Marcondes Acta Cir Bras Original Article PURPOSE: Herein we evaluated the effects of platelet concentrate (PC) and platelet-poor plasma (PPP) on bone repair using noncritical defects in the calvaria of rabbits and compared them to the presence of TGF-β1 and osteocalcin on reparative sites. METHODS: Five noncritical defects of 8.7 mm in diameter were created on the calvaria of 15 animals. Each defect was treated differently, using autograft (ABG), ABG associated with PC (ABG + PC), ABG with PPP (ABG + PPP), isolated PPP, and blood clot (control). The animals were submitted to euthanasia on the second, fourth and sixth week post-surgery. RESULTS: The defects that received ABG+PC or PPP demonstrated lower bone formation when compared to specimens that received ABG in the same period. These results coincided to significant higher immunopositivity for TGF-β1 for specimens that received PC, and lower presence of cytokine in the group PPP. However, either higher or lower presence of TGF-β1 were also correlated to lower presence of osteocalcin. Likewise, these results were similar to findings in specimens treated only with PPP when compared to control. CONCLUSIONS: PC and PPP were not effective when applied in association with ABG. Similarly, isolated use of PPP was not beneficial in optimizing the bone repair. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia 2021-07-19 /pmc/articles/PMC8291903/ /pubmed/34287607 http://dx.doi.org/10.1590/ACB360604 Text en https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
de Oliveira-Filho, Marco Antonio
Souza, Marcelo
Tabushi, Fernando Issamu
Almeida, Luís Eduardo
Figueiredo, Fernanda Pirajá
Lourenço, Elora Sampaio
Giovanini, Allan Fernando
Malafaia, Osvaldo
Ribas, Jurandir Marcondes
Platelet-concentrated and platelet poor-plasma promote different pattern on immunohistochemical expression of TGF-β1, however they impairs the osteoneogensis in calvarial defects treated with autograft due suppression of osteocalcin
title Platelet-concentrated and platelet poor-plasma promote different pattern on immunohistochemical expression of TGF-β1, however they impairs the osteoneogensis in calvarial defects treated with autograft due suppression of osteocalcin
title_full Platelet-concentrated and platelet poor-plasma promote different pattern on immunohistochemical expression of TGF-β1, however they impairs the osteoneogensis in calvarial defects treated with autograft due suppression of osteocalcin
title_fullStr Platelet-concentrated and platelet poor-plasma promote different pattern on immunohistochemical expression of TGF-β1, however they impairs the osteoneogensis in calvarial defects treated with autograft due suppression of osteocalcin
title_full_unstemmed Platelet-concentrated and platelet poor-plasma promote different pattern on immunohistochemical expression of TGF-β1, however they impairs the osteoneogensis in calvarial defects treated with autograft due suppression of osteocalcin
title_short Platelet-concentrated and platelet poor-plasma promote different pattern on immunohistochemical expression of TGF-β1, however they impairs the osteoneogensis in calvarial defects treated with autograft due suppression of osteocalcin
title_sort platelet-concentrated and platelet poor-plasma promote different pattern on immunohistochemical expression of tgf-β1, however they impairs the osteoneogensis in calvarial defects treated with autograft due suppression of osteocalcin
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8291903/
https://www.ncbi.nlm.nih.gov/pubmed/34287607
http://dx.doi.org/10.1590/ACB360604
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