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Efficient gene transfer into zebra finch germline-competent stem cells using an adenoviral vector system

Zebra finch is a representative animal model for studying the molecular basis of human disorders of vocal development and communication. Accordingly, various functional studies of zebra finch have knocked down or introduced foreign genes in vivo; however, their germline transmission efficiency is re...

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Autores principales: Jung, Kyung Min, Kim, Young Min, Kim, Jin Lee, Han, Jae Yong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8292312/
https://www.ncbi.nlm.nih.gov/pubmed/34285320
http://dx.doi.org/10.1038/s41598-021-94229-x
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author Jung, Kyung Min
Kim, Young Min
Kim, Jin Lee
Han, Jae Yong
author_facet Jung, Kyung Min
Kim, Young Min
Kim, Jin Lee
Han, Jae Yong
author_sort Jung, Kyung Min
collection PubMed
description Zebra finch is a representative animal model for studying the molecular basis of human disorders of vocal development and communication. Accordingly, various functional studies of zebra finch have knocked down or introduced foreign genes in vivo; however, their germline transmission efficiency is remarkably low. The primordial germ cell (PGC)-mediated method is preferred for avian transgenic studies; however, use of this method is restricted in zebra finch due to the lack of an efficient gene transfer method for the germline. To target primary germ cells that are difficult to transfect and manipulate, an adenovirus-mediated gene transfer system with high efficiency in a wide range of cell types may be useful. Here, we isolated and characterized two types of primary germline-competent stem cells, PGCs and spermatogonial stem cells (SSCs), from embryonic and adult reproductive tissues of zebra finch and demonstrated that genes were most efficiently transferred into these cells using an adenovirus-mediated system. This system was successfully used to generate gene-edited PGCs in vitro. These results are expected to improve transgenic zebra finch production.
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spelling pubmed-82923122021-07-21 Efficient gene transfer into zebra finch germline-competent stem cells using an adenoviral vector system Jung, Kyung Min Kim, Young Min Kim, Jin Lee Han, Jae Yong Sci Rep Article Zebra finch is a representative animal model for studying the molecular basis of human disorders of vocal development and communication. Accordingly, various functional studies of zebra finch have knocked down or introduced foreign genes in vivo; however, their germline transmission efficiency is remarkably low. The primordial germ cell (PGC)-mediated method is preferred for avian transgenic studies; however, use of this method is restricted in zebra finch due to the lack of an efficient gene transfer method for the germline. To target primary germ cells that are difficult to transfect and manipulate, an adenovirus-mediated gene transfer system with high efficiency in a wide range of cell types may be useful. Here, we isolated and characterized two types of primary germline-competent stem cells, PGCs and spermatogonial stem cells (SSCs), from embryonic and adult reproductive tissues of zebra finch and demonstrated that genes were most efficiently transferred into these cells using an adenovirus-mediated system. This system was successfully used to generate gene-edited PGCs in vitro. These results are expected to improve transgenic zebra finch production. Nature Publishing Group UK 2021-07-20 /pmc/articles/PMC8292312/ /pubmed/34285320 http://dx.doi.org/10.1038/s41598-021-94229-x Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Jung, Kyung Min
Kim, Young Min
Kim, Jin Lee
Han, Jae Yong
Efficient gene transfer into zebra finch germline-competent stem cells using an adenoviral vector system
title Efficient gene transfer into zebra finch germline-competent stem cells using an adenoviral vector system
title_full Efficient gene transfer into zebra finch germline-competent stem cells using an adenoviral vector system
title_fullStr Efficient gene transfer into zebra finch germline-competent stem cells using an adenoviral vector system
title_full_unstemmed Efficient gene transfer into zebra finch germline-competent stem cells using an adenoviral vector system
title_short Efficient gene transfer into zebra finch germline-competent stem cells using an adenoviral vector system
title_sort efficient gene transfer into zebra finch germline-competent stem cells using an adenoviral vector system
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8292312/
https://www.ncbi.nlm.nih.gov/pubmed/34285320
http://dx.doi.org/10.1038/s41598-021-94229-x
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