Efficient Protoplast Regeneration Protocol and CRISPR/Cas9-Mediated Editing of Glucosinolate Transporter (GTR) Genes in Rapeseed (Brassica napus L.)

Difficulty in protoplast regeneration is a major obstacle to apply the CRISPR/Cas9 gene editing technique effectively in research and breeding of rapeseed (Brassica napus L.). The present study describes for the first time a rapid and efficient protocol for the isolation, regeneration and transfecti...

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Detalles Bibliográficos
Autores principales: Li, Xueyuan, Sandgrind, Sjur, Moss, Oliver, Guan, Rui, Ivarson, Emelie, Wang, Eu Sheng, Kanagarajan, Selvaraju, Zhu, Li-Hua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Plant Science
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8294089/
https://www.ncbi.nlm.nih.gov/pubmed/34305978
http://dx.doi.org/10.3389/fpls.2021.680859

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8294089/
https://www.ncbi.nlm.nih.gov/pubmed/34305978
http://dx.doi.org/10.3389/fpls.2021.680859

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