Co-Immobilized Capillary Enzyme Reactor Based on Beta-Secretase1 and Acetylcholinesterase: A Model for Dual-Ligand Screening

We have developed a dual enzymatic system assay involving liquid chromatography-mass spectrometry (LC–MS) to screen AChE and BACE1 ligands. A fused silica capillary (30 cm × 0.1 mm i.d. × 0.362 mm e.d.) was used as solid support. The co-immobilization procedure encompassed two steps and random immob...

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Detalles Bibliográficos
Autores principales: Vilela, Adriana Ferreira Lopes, Narciso dos Reis, Vitor Eduardo, Cardoso, Carmen Lúcia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8295500/
https://www.ncbi.nlm.nih.gov/pubmed/34307303
http://dx.doi.org/10.3389/fchem.2021.708374

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8295500/
https://www.ncbi.nlm.nih.gov/pubmed/34307303
http://dx.doi.org/10.3389/fchem.2021.708374

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