Cargando…

Single nucleotide replacement in the Atlantic salmon genome using CRISPR/Cas9 and asymmetrical oligonucleotide donors

BACKGROUND: New breeding technologies (NBT) using CRISPR/Cas9-induced homology directed repair (HDR) has the potential to expedite genetic improvement in aquaculture. The long generation time in Atlantic salmon makes breeding an unattractive solution to obtain homozygous mutants and improving the ra...

Descripción completa

Detalles Bibliográficos
Autores principales:	Straume, Anne Hege, Kjærner-Semb, Erik, Skaftnesmo, Kai Ove, Güralp, Hilal, Lillico, Simon, Wargelius, Anna, Edvardsen, Rolf Brudvik
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2021
Materias:	Research
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8296724/ https://www.ncbi.nlm.nih.gov/pubmed/34294050 http://dx.doi.org/10.1186/s12864-021-07823-8

Ejemplares similares

Indel locations are determined by template polarity in highly efficient in vivo CRISPR/Cas9-mediated HDR in Atlantic salmon
por: Straume, Anne Hege, et al.
Publicado: (2020)

Rescue of germ cells in dnd crispant embryos opens the possibility to produce inherited sterility in Atlantic salmon
por: Güralp, Hilal, et al.
Publicado: (2020)

Author Correction: Rescue of germ cells in dnd crispant embryos opens the possibility to produce inherited sterility in Atlantic salmon
por: Güralp, Hilal, et al.
Publicado: (2021)

Targeted Mutagenesis in Atlantic Salmon (Salmo salar L.) Using the CRISPR/Cas9 System Induces Complete Knockout Individuals in the F0 Generation
por: Edvardsen, Rolf B., et al.
Publicado: (2014)

Transcriptomic analysis of dead end knockout testis reveals germ cell and gonadal somatic factors in Atlantic salmon
por: Kleppe, Lene, et al.
Publicado: (2020)

Pituitary Gonadotropin Gene Expression During Induced Onset of Postsmolt Maturation in Male Atlantic Salmon: In Vivo and Tissue Culture Studies
por: Crespo, Diego, et al.
Publicado: (2022)

Vgll3 and the Hippo pathway are regulated in Sertoli cells upon entry and during puberty in Atlantic salmon testis
por: Kjærner-Semb, Erik, et al.
Publicado: (2018)

The influence of vgll3 genotypes on sea age at maturity is altered in farmed mowi strain Atlantic salmon
por: Ayllon, Fernando, et al.
Publicado: (2019)

Dnd knockout ablates germ cells and demonstrates germ cell independent sex differentiation in Atlantic salmon
por: Wargelius, Anna, et al.
Publicado: (2016)

Atlantic salmon populations reveal adaptive divergence of immune related genes - a duplicated genome under selection
por: Kjærner-Semb, Erik, et al.
Publicado: (2016)

Comparison of anadromous and landlocked Atlantic salmon genomes reveals signatures of parallel and relaxed selection across the Northern Hemisphere
por: Kjærner‐Semb, Erik, et al.
Publicado: (2020)

The vgll3 Locus Controls Age at Maturity in Wild and Domesticated Atlantic Salmon (Salmo salar L.) Males
por: Ayllon, Fernando, et al.
Publicado: (2015)

The Piwil1 N domain is required for germ cell survival in Atlantic salmon
por: F. L, Almeida, et al.
Publicado: (2022)

Autosomal sdY Pseudogenes Explain Discordances Between Phenotypic Sex and DNA Marker for Sex Identification in Atlantic Salmon
por: Ayllon, Fernando, et al.
Publicado: (2020)

Loss of stra8 Increases Germ Cell Apoptosis but Is Still Compatible With Sperm Production in Atlantic Salmon (Salmo salar)
por: Skaftnesmo, Kai O., et al.
Publicado: (2021)

Integrative testis transcriptome analysis reveals differentially expressed miRNAs and their mRNA targets during early puberty in Atlantic salmon
por: Skaftnesmo, K. O., et al.
Publicado: (2017)

Sex steroid production associated with puberty is absent in germ cell-free salmon
por: Kleppe, Lene, et al.
Publicado: (2017)

Global transcriptome analysis identifies regulated transcripts and pathways activated during oogenesis and early embryogenesis in atlantic cod
por: Kleppe, Lene, et al.
Publicado: (2014)

CRISPR/Cas9-mediated editing of Δ5 and Δ6 desaturases impairs Δ8-desaturation and docosahexaenoic acid synthesis in Atlantic salmon (Salmo salar L.)
por: Datsomor, Alex K., et al.
Publicado: (2019)

CRISPR/Cas9-mediated ablation of elovl2 in Atlantic salmon (Salmo salar L.) inhibits elongation of polyunsaturated fatty acids and induces Srebp-1 and target genes
por: Datsomor, Alex K., et al.
Publicado: (2019)

Impact of Antioxidant Feed and Growth Manipulation on the Redox Regulation of Atlantic Salmon Smolts
por: Yin, Peng, et al.
Publicado: (2022)

Heterochiasmy and the establishment of gsdf as a novel sex determining gene in Atlantic halibut
por: Edvardsen, Rolf Brudvik, et al.
Publicado: (2022)

Targeted mutagenesis of ∆5 and ∆6 fatty acyl desaturases induce dysregulation of lipid metabolism in Atlantic salmon (Salmo salar)
por: Jin, Yang, et al.
Publicado: (2020)

Development of supermale and all-male Atlantic salmon to research the vgll3 allele - puberty link
por: Fjelldal, Per Gunnar, et al.
Publicado: (2020)

CpG oligonucleotides bind TLR9 and RRM-Containing proteins in Atlantic Salmon (Salmo salar)
por: Iliev, Dimitar B, et al.
Publicado: (2013)

Extracting functionally accurate context-specific models of Atlantic salmon metabolism
por: Molversmyr, Håvard, et al.
Publicado: (2023)

Carotenoid dynamics in Atlantic salmon
por: Rajasingh, Hannah, et al.
Publicado: (2006)

Molecular Evidence that Only Two Opsin Subfamilies, the Blue Light- (SWS2) and Green Light-Sensitive (RH2), Drive Color Vision in Atlantic Cod (Gadus morhua)
por: Valen, Ragnhild, et al.
Publicado: (2014)

Transcriptome sequencing of Atlantic salmon (Salmo salar L.) notochord prior to development of the vertebrae provides clues to regulation of positional fate, chordoblast lineage and mineralisation
por: Wang, Shou, et al.
Publicado: (2014)

Single nucleotide polymorphisms to discriminate different classes of hybrid between wild Atlantic salmon and aquaculture escapees
por: Pritchard, Victoria L., et al.
Publicado: (2016)

Maternal 3’UTRs: from egg to onset of zygotic transcription in Atlantic cod
por: Kleppe, Lene, et al.
Publicado: (2012)

Redefining the oceanic distribution of Atlantic salmon
por: Rikardsen, Audun H., et al.
Publicado: (2021)

Bacterial networks in Atlantic salmon with Piscirickettsiosis
por: Coca, Yoandy, et al.
Publicado: (2023)

Infectious salmon anaemia virus (ISAV) mucosal infection in Atlantic salmon
por: Aamelfot, Maria, et al.
Publicado: (2015)

SALARECON connects the Atlantic salmon genome to growth and feed efficiency
por: Zakhartsev, Maksim, et al.
Publicado: (2022)

Atlantic Salmon Reovirus Infection Causes a CD8 T Cell Myocarditis in Atlantic Salmon (Salmo salar L.)
por: Mikalsen, Aase B., et al.
Publicado: (2012)

Demonstration of infectious salmon anaemia virus (ISAV) endocytosis in erythrocytes of Atlantic salmon
por: Workenhe, Samuel T, et al.
Publicado: (2007)

Infectious salmon anaemia virus infection of Atlantic salmon gill epithelial cells
por: Weli, Simon Chioma, et al.
Publicado: (2013)

Effects of laboratory salmon louse infection on osmoregulation, growth and survival in Atlantic salmon
por: Fjelldal, Per Gunnar, et al.
Publicado: (2020)

IgM+ and IgT+ B Cell Traffic to the Heart during SAV Infection in Atlantic Salmon
por: Bakke, Anne Flore, et al.
Publicado: (2020)

Cannot write session to /tmp/vufind_sessions/sess_7co0it18uvc6o7iu0bari6et1q