Cargando…

Blood Pressure-Lowering Effect of Wine Lees Phenolic Compounds Is Mediated by Endothelial-Derived Factors: Role of Sirtuin 1

The antihypertensive effect of wine lees powder (WLPW) from a Cabernet grape variety was related to its high content in flavanols and anthocyanins compounds. This study investigates the involvement of endothelial-derived factors and SIRT1 in its bioactivity. Spontaneously hypertensive rats (SHR) wer...

Descripción completa

Detalles Bibliográficos
Autores principales: López-Fernández-Sobrino, Raúl, Soliz-Rueda, Jorge R., Ávila-Román, Javier, Arola-Arnal, Anna, Suárez, Manuel, Muguerza, Begoña, Bravo, Francisca Isabel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8301028/
https://www.ncbi.nlm.nih.gov/pubmed/34356306
http://dx.doi.org/10.3390/antiox10071073
_version_ 1783726576872980480
author López-Fernández-Sobrino, Raúl
Soliz-Rueda, Jorge R.
Ávila-Román, Javier
Arola-Arnal, Anna
Suárez, Manuel
Muguerza, Begoña
Bravo, Francisca Isabel
author_facet López-Fernández-Sobrino, Raúl
Soliz-Rueda, Jorge R.
Ávila-Román, Javier
Arola-Arnal, Anna
Suárez, Manuel
Muguerza, Begoña
Bravo, Francisca Isabel
author_sort López-Fernández-Sobrino, Raúl
collection PubMed
description The antihypertensive effect of wine lees powder (WLPW) from a Cabernet grape variety was related to its high content in flavanols and anthocyanins compounds. This study investigates the involvement of endothelial-derived factors and SIRT1 in its bioactivity. Spontaneously hypertensive rats (SHR) were orally administered water or WLPW (125 mg/kg bw). Posteriorly, both groups were intraperitoneally administered saline, Nω-nitro-L-arginine methyl ester (L-NAME), a nitric oxide (NO) synthesis inhibitor, indomethacin, a prostacyclin synthesis inhibitor, or sirtinol, an inhibitor of sirtuins. Blood pressure (BP) was recorded before and 6 h after WLPW administration. In an additional experiment, SHR were administered water or WLPW and endothelial expressions of eNos, Sirt1, Nox4, and Et1 were determined. The BP-lowering properties of WLPW were abolished by L-NAME and partially reduced by indomethacin, demonstrating that WLPW antihypertensive effect was mediated by changes in NO availability, although prostacyclin also contributed to this activity. Moreover, BP-lowering effect was reduced by sirtinol, indicating that WLPW decreased BP in a SIRT1-dependent manner. Furthermore, WLPW upregulated eNos and Sirt1 and downregulated Nox4 and Et1 endothelial gene expression. These results evidence the vasoprotective effect of WLPW and show that its antihypertensive effect in SHR is endothelium dependent and mediated by SIRT1.
format Online
Article
Text
id pubmed-8301028
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-83010282021-07-24 Blood Pressure-Lowering Effect of Wine Lees Phenolic Compounds Is Mediated by Endothelial-Derived Factors: Role of Sirtuin 1 López-Fernández-Sobrino, Raúl Soliz-Rueda, Jorge R. Ávila-Román, Javier Arola-Arnal, Anna Suárez, Manuel Muguerza, Begoña Bravo, Francisca Isabel Antioxidants (Basel) Article The antihypertensive effect of wine lees powder (WLPW) from a Cabernet grape variety was related to its high content in flavanols and anthocyanins compounds. This study investigates the involvement of endothelial-derived factors and SIRT1 in its bioactivity. Spontaneously hypertensive rats (SHR) were orally administered water or WLPW (125 mg/kg bw). Posteriorly, both groups were intraperitoneally administered saline, Nω-nitro-L-arginine methyl ester (L-NAME), a nitric oxide (NO) synthesis inhibitor, indomethacin, a prostacyclin synthesis inhibitor, or sirtinol, an inhibitor of sirtuins. Blood pressure (BP) was recorded before and 6 h after WLPW administration. In an additional experiment, SHR were administered water or WLPW and endothelial expressions of eNos, Sirt1, Nox4, and Et1 were determined. The BP-lowering properties of WLPW were abolished by L-NAME and partially reduced by indomethacin, demonstrating that WLPW antihypertensive effect was mediated by changes in NO availability, although prostacyclin also contributed to this activity. Moreover, BP-lowering effect was reduced by sirtinol, indicating that WLPW decreased BP in a SIRT1-dependent manner. Furthermore, WLPW upregulated eNos and Sirt1 and downregulated Nox4 and Et1 endothelial gene expression. These results evidence the vasoprotective effect of WLPW and show that its antihypertensive effect in SHR is endothelium dependent and mediated by SIRT1. MDPI 2021-07-03 /pmc/articles/PMC8301028/ /pubmed/34356306 http://dx.doi.org/10.3390/antiox10071073 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
López-Fernández-Sobrino, Raúl
Soliz-Rueda, Jorge R.
Ávila-Román, Javier
Arola-Arnal, Anna
Suárez, Manuel
Muguerza, Begoña
Bravo, Francisca Isabel
Blood Pressure-Lowering Effect of Wine Lees Phenolic Compounds Is Mediated by Endothelial-Derived Factors: Role of Sirtuin 1
title Blood Pressure-Lowering Effect of Wine Lees Phenolic Compounds Is Mediated by Endothelial-Derived Factors: Role of Sirtuin 1
title_full Blood Pressure-Lowering Effect of Wine Lees Phenolic Compounds Is Mediated by Endothelial-Derived Factors: Role of Sirtuin 1
title_fullStr Blood Pressure-Lowering Effect of Wine Lees Phenolic Compounds Is Mediated by Endothelial-Derived Factors: Role of Sirtuin 1
title_full_unstemmed Blood Pressure-Lowering Effect of Wine Lees Phenolic Compounds Is Mediated by Endothelial-Derived Factors: Role of Sirtuin 1
title_short Blood Pressure-Lowering Effect of Wine Lees Phenolic Compounds Is Mediated by Endothelial-Derived Factors: Role of Sirtuin 1
title_sort blood pressure-lowering effect of wine lees phenolic compounds is mediated by endothelial-derived factors: role of sirtuin 1
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8301028/
https://www.ncbi.nlm.nih.gov/pubmed/34356306
http://dx.doi.org/10.3390/antiox10071073
work_keys_str_mv AT lopezfernandezsobrinoraul bloodpressureloweringeffectofwineleesphenoliccompoundsismediatedbyendothelialderivedfactorsroleofsirtuin1
AT solizruedajorger bloodpressureloweringeffectofwineleesphenoliccompoundsismediatedbyendothelialderivedfactorsroleofsirtuin1
AT avilaromanjavier bloodpressureloweringeffectofwineleesphenoliccompoundsismediatedbyendothelialderivedfactorsroleofsirtuin1
AT arolaarnalanna bloodpressureloweringeffectofwineleesphenoliccompoundsismediatedbyendothelialderivedfactorsroleofsirtuin1
AT suarezmanuel bloodpressureloweringeffectofwineleesphenoliccompoundsismediatedbyendothelialderivedfactorsroleofsirtuin1
AT muguerzabegona bloodpressureloweringeffectofwineleesphenoliccompoundsismediatedbyendothelialderivedfactorsroleofsirtuin1
AT bravofranciscaisabel bloodpressureloweringeffectofwineleesphenoliccompoundsismediatedbyendothelialderivedfactorsroleofsirtuin1