Cargando…
Soybean and Lupine Addition in Hen Nutrition—Influence on Egg Immunoreactivity
Modifying hen fodder is a common way of changing eggs composition today. However, there is no information on the effect of the source of protein in the fodder replacement on egg allergenicity. This research aimed to detect potential differences in the immunoreactivity and protein composition of eggs...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8305273/ https://www.ncbi.nlm.nih.gov/pubmed/34299594 http://dx.doi.org/10.3390/molecules26144319 |
_version_ | 1783727534944288768 |
---|---|
author | Tomczak, Aneta Misiak, Michalina Zielińska-Dawidziak, Magdalena |
author_facet | Tomczak, Aneta Misiak, Michalina Zielińska-Dawidziak, Magdalena |
author_sort | Tomczak, Aneta |
collection | PubMed |
description | Modifying hen fodder is a common way of changing eggs composition today. However, there is no information on the effect of the source of protein in the fodder replacement on egg allergenicity. This research aimed to detect potential differences in the immunoreactivity and protein composition of eggs from hens fed with fodder containing legume. The aim of the first step of the study was to select the proper solvent for extracting allergenic proteins from hen eggs. Two of them (containing Tween 20 and Triton 100) were selected, based on protein profile and concentration analysis. Egg-white- and egg-yolk-proteins extracts prepared with them were checked for potential differences, using SDS-PAGE electrophoresis, and then the Western-blot method, using sera from children allergic to eggs and soy. Preliminary studies on the influence of fodder composition on the composition of egg proteins suggest that the addition of soy and lupine to fodder modifies the expression of egg proteins. The observed differences in the immunoreactivity of proteins contained in hen egg-white samples do not seem to be as significant as the appearance of protein with a molecular weight of ~13 kDa in the yolk of eggs obtained from soybean-fed hens. This protein may increase the immunoreactivity of eggs for children allergic solely to soy. |
format | Online Article Text |
id | pubmed-8305273 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-83052732021-07-25 Soybean and Lupine Addition in Hen Nutrition—Influence on Egg Immunoreactivity Tomczak, Aneta Misiak, Michalina Zielińska-Dawidziak, Magdalena Molecules Article Modifying hen fodder is a common way of changing eggs composition today. However, there is no information on the effect of the source of protein in the fodder replacement on egg allergenicity. This research aimed to detect potential differences in the immunoreactivity and protein composition of eggs from hens fed with fodder containing legume. The aim of the first step of the study was to select the proper solvent for extracting allergenic proteins from hen eggs. Two of them (containing Tween 20 and Triton 100) were selected, based on protein profile and concentration analysis. Egg-white- and egg-yolk-proteins extracts prepared with them were checked for potential differences, using SDS-PAGE electrophoresis, and then the Western-blot method, using sera from children allergic to eggs and soy. Preliminary studies on the influence of fodder composition on the composition of egg proteins suggest that the addition of soy and lupine to fodder modifies the expression of egg proteins. The observed differences in the immunoreactivity of proteins contained in hen egg-white samples do not seem to be as significant as the appearance of protein with a molecular weight of ~13 kDa in the yolk of eggs obtained from soybean-fed hens. This protein may increase the immunoreactivity of eggs for children allergic solely to soy. MDPI 2021-07-16 /pmc/articles/PMC8305273/ /pubmed/34299594 http://dx.doi.org/10.3390/molecules26144319 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Tomczak, Aneta Misiak, Michalina Zielińska-Dawidziak, Magdalena Soybean and Lupine Addition in Hen Nutrition—Influence on Egg Immunoreactivity |
title | Soybean and Lupine Addition in Hen Nutrition—Influence on Egg Immunoreactivity |
title_full | Soybean and Lupine Addition in Hen Nutrition—Influence on Egg Immunoreactivity |
title_fullStr | Soybean and Lupine Addition in Hen Nutrition—Influence on Egg Immunoreactivity |
title_full_unstemmed | Soybean and Lupine Addition in Hen Nutrition—Influence on Egg Immunoreactivity |
title_short | Soybean and Lupine Addition in Hen Nutrition—Influence on Egg Immunoreactivity |
title_sort | soybean and lupine addition in hen nutrition—influence on egg immunoreactivity |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8305273/ https://www.ncbi.nlm.nih.gov/pubmed/34299594 http://dx.doi.org/10.3390/molecules26144319 |
work_keys_str_mv | AT tomczakaneta soybeanandlupineadditioninhennutritioninfluenceoneggimmunoreactivity AT misiakmichalina soybeanandlupineadditioninhennutritioninfluenceoneggimmunoreactivity AT zielinskadawidziakmagdalena soybeanandlupineadditioninhennutritioninfluenceoneggimmunoreactivity |