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The Effect of Genomic DNA Contamination on the Detection of Circulating Long Non-Coding RNAs: The Paradigm of MALAT1

The presence of contaminating gDNA in RNA preparations is a frequent cause of false positives in RT-PCR-based analysis. However, in some cases, this cannot be avoided, especially when there are no exons–intron junctions in the lncRNA sequences. Due to the lack of exons in few of long noncoding RNAs...

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Detalles Bibliográficos
Autores principales: Markou, Athina N., Smilkou, Stavroula, Tsaroucha, Emilia, Lianidou, Evi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8305527/
https://www.ncbi.nlm.nih.gov/pubmed/34202021
http://dx.doi.org/10.3390/diagnostics11071160