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Chromenol Derivatives as Novel Antifungal Agents: Synthesis, In Silico and In Vitro Evaluation

Herein we report the synthesis of some new 1H-1,2,4-triazole functionalized chromenols (3a–3n) via tandem reactions of 1-(alkyl/aryl)-2-(1H-1,2,4-triazole-1-yl) with salicylic aldehydes and the evaluation of their antifungal activity. In silico prediction of biological activity with computer program...

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Autores principales: Zveaghintseva, Marina, Stingaci, Eugenia, Pogrebnoi, Serghei, Smetanscaia, Anastasia, Valica, Vladimir, Uncu, Livia, Ch. Kravtsov, Victor, Melnic, Elena, Petrou, Anthi, Glamočlija, Jasmina, Soković, Marina, Carazo, Alejandro, Mladěnka, Přemysl, Poroikov, Vladimir, Geronikaki, Athina, Macaev, Fliur Z.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8307147/
https://www.ncbi.nlm.nih.gov/pubmed/34299579
http://dx.doi.org/10.3390/molecules26144304
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author Zveaghintseva, Marina
Stingaci, Eugenia
Pogrebnoi, Serghei
Smetanscaia, Anastasia
Valica, Vladimir
Uncu, Livia
Ch. Kravtsov, Victor
Melnic, Elena
Petrou, Anthi
Glamočlija, Jasmina
Soković, Marina
Carazo, Alejandro
Mladěnka, Přemysl
Poroikov, Vladimir
Geronikaki, Athina
Macaev, Fliur Z.
author_facet Zveaghintseva, Marina
Stingaci, Eugenia
Pogrebnoi, Serghei
Smetanscaia, Anastasia
Valica, Vladimir
Uncu, Livia
Ch. Kravtsov, Victor
Melnic, Elena
Petrou, Anthi
Glamočlija, Jasmina
Soković, Marina
Carazo, Alejandro
Mladěnka, Přemysl
Poroikov, Vladimir
Geronikaki, Athina
Macaev, Fliur Z.
author_sort Zveaghintseva, Marina
collection PubMed
description Herein we report the synthesis of some new 1H-1,2,4-triazole functionalized chromenols (3a–3n) via tandem reactions of 1-(alkyl/aryl)-2-(1H-1,2,4-triazole-1-yl) with salicylic aldehydes and the evaluation of their antifungal activity. In silico prediction of biological activity with computer program PASS indicate that the compounds have a high novelty compared to the known antifungal agents. We did not find any close analog among the over 580,000 pharmaceutical agents in the Cortellis Drug Discovery Intelligence database at the similarity cutoff of 70%. The evaluation of antifungal activity in vitro revealed that the highest activity was exhibited by compound 3k, followed by 3n. Their MIC values for different fungi were 22.1–184.2 and 71.3–199.8 µM, respectively. Twelve from fourteen tested compounds were more active than the reference drugs ketoconazole and bifonazole. The most sensitive fungus appeared to be Trichoderma viride, while Aspergillus fumigatus was the most resistant one. It was found that the presence of the 2-(tert-butyl)-2H-chromen-2-ol substituent on the 4th position of the triazole ring is very beneficial for antifungal activity. Molecular docking studies on C. albicans sterol 14α-demethylase (CYP51) and DNA topoisomerase IV were used to predict the mechanism of antifungal activities. According to the docking results, the inhibition of CYP51 is a putative mechanism of antifungal activity of the novel chromenol derivatives. We also showed that most active compounds have a low cytotoxicity, which allows us to consider them promising antifungal agents for the subsequent testing activity in in vivo assays.
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spelling pubmed-83071472021-07-25 Chromenol Derivatives as Novel Antifungal Agents: Synthesis, In Silico and In Vitro Evaluation Zveaghintseva, Marina Stingaci, Eugenia Pogrebnoi, Serghei Smetanscaia, Anastasia Valica, Vladimir Uncu, Livia Ch. Kravtsov, Victor Melnic, Elena Petrou, Anthi Glamočlija, Jasmina Soković, Marina Carazo, Alejandro Mladěnka, Přemysl Poroikov, Vladimir Geronikaki, Athina Macaev, Fliur Z. Molecules Article Herein we report the synthesis of some new 1H-1,2,4-triazole functionalized chromenols (3a–3n) via tandem reactions of 1-(alkyl/aryl)-2-(1H-1,2,4-triazole-1-yl) with salicylic aldehydes and the evaluation of their antifungal activity. In silico prediction of biological activity with computer program PASS indicate that the compounds have a high novelty compared to the known antifungal agents. We did not find any close analog among the over 580,000 pharmaceutical agents in the Cortellis Drug Discovery Intelligence database at the similarity cutoff of 70%. The evaluation of antifungal activity in vitro revealed that the highest activity was exhibited by compound 3k, followed by 3n. Their MIC values for different fungi were 22.1–184.2 and 71.3–199.8 µM, respectively. Twelve from fourteen tested compounds were more active than the reference drugs ketoconazole and bifonazole. The most sensitive fungus appeared to be Trichoderma viride, while Aspergillus fumigatus was the most resistant one. It was found that the presence of the 2-(tert-butyl)-2H-chromen-2-ol substituent on the 4th position of the triazole ring is very beneficial for antifungal activity. Molecular docking studies on C. albicans sterol 14α-demethylase (CYP51) and DNA topoisomerase IV were used to predict the mechanism of antifungal activities. According to the docking results, the inhibition of CYP51 is a putative mechanism of antifungal activity of the novel chromenol derivatives. We also showed that most active compounds have a low cytotoxicity, which allows us to consider them promising antifungal agents for the subsequent testing activity in in vivo assays. MDPI 2021-07-16 /pmc/articles/PMC8307147/ /pubmed/34299579 http://dx.doi.org/10.3390/molecules26144304 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zveaghintseva, Marina
Stingaci, Eugenia
Pogrebnoi, Serghei
Smetanscaia, Anastasia
Valica, Vladimir
Uncu, Livia
Ch. Kravtsov, Victor
Melnic, Elena
Petrou, Anthi
Glamočlija, Jasmina
Soković, Marina
Carazo, Alejandro
Mladěnka, Přemysl
Poroikov, Vladimir
Geronikaki, Athina
Macaev, Fliur Z.
Chromenol Derivatives as Novel Antifungal Agents: Synthesis, In Silico and In Vitro Evaluation
title Chromenol Derivatives as Novel Antifungal Agents: Synthesis, In Silico and In Vitro Evaluation
title_full Chromenol Derivatives as Novel Antifungal Agents: Synthesis, In Silico and In Vitro Evaluation
title_fullStr Chromenol Derivatives as Novel Antifungal Agents: Synthesis, In Silico and In Vitro Evaluation
title_full_unstemmed Chromenol Derivatives as Novel Antifungal Agents: Synthesis, In Silico and In Vitro Evaluation
title_short Chromenol Derivatives as Novel Antifungal Agents: Synthesis, In Silico and In Vitro Evaluation
title_sort chromenol derivatives as novel antifungal agents: synthesis, in silico and in vitro evaluation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8307147/
https://www.ncbi.nlm.nih.gov/pubmed/34299579
http://dx.doi.org/10.3390/molecules26144304
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