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Mycobiota in the Carposphere of Sour and Sweet Cherries and Antagonistic Features of Potential Biocontrol Yeasts

Sour cherries (Prunus cerasus L.) and sweet cherries (P. avium L.) are economically important fruits with high potential in the food industry and medicine. In this study, we analyzed fungal communities associated with the carposphere of sour and sweet cherries that were freshly harvested from privat...

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Autores principales: Stanevičienė, Ramunė, Lukša, Juliana, Strazdaitė-Žielienė, Živilė, Ravoitytė, Bazilė, Losinska-Sičiūnienė, Regina, Mozūraitis, Raimondas, Servienė, Elena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8307871/
https://www.ncbi.nlm.nih.gov/pubmed/34209423
http://dx.doi.org/10.3390/microorganisms9071423
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author Stanevičienė, Ramunė
Lukša, Juliana
Strazdaitė-Žielienė, Živilė
Ravoitytė, Bazilė
Losinska-Sičiūnienė, Regina
Mozūraitis, Raimondas
Servienė, Elena
author_facet Stanevičienė, Ramunė
Lukša, Juliana
Strazdaitė-Žielienė, Živilė
Ravoitytė, Bazilė
Losinska-Sičiūnienė, Regina
Mozūraitis, Raimondas
Servienė, Elena
author_sort Stanevičienė, Ramunė
collection PubMed
description Sour cherries (Prunus cerasus L.) and sweet cherries (P. avium L.) are economically important fruits with high potential in the food industry and medicine. In this study, we analyzed fungal communities associated with the carposphere of sour and sweet cherries that were freshly harvested from private plantations and purchased in a food store. Following DNA isolation, a DNA fragment of the ITS2 rRNA gene region of each sample was individually amplified and subjected to high-throughput NGS sequencing. Analysis of 168,933 high-quality reads showed the presence of 690 fungal taxa. Investigation of microbial ASVs diversity revealed plant-dependent and postharvest handling-affected fungal assemblages. Among the microorganisms inhabiting tested berries, potentially beneficial or pathogenic fungi were documented. Numerous cultivable yeasts were isolated from the surface of tested berries and characterized by their antagonistic activity. Some of the isolates, identified as Aureobasidium pullulans, Metschnikowia fructicola, and M. pulcherrima, displayed pronounced activity against potential fungal pathogens and showed attractiveness for disease control.
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spelling pubmed-83078712021-07-25 Mycobiota in the Carposphere of Sour and Sweet Cherries and Antagonistic Features of Potential Biocontrol Yeasts Stanevičienė, Ramunė Lukša, Juliana Strazdaitė-Žielienė, Živilė Ravoitytė, Bazilė Losinska-Sičiūnienė, Regina Mozūraitis, Raimondas Servienė, Elena Microorganisms Article Sour cherries (Prunus cerasus L.) and sweet cherries (P. avium L.) are economically important fruits with high potential in the food industry and medicine. In this study, we analyzed fungal communities associated with the carposphere of sour and sweet cherries that were freshly harvested from private plantations and purchased in a food store. Following DNA isolation, a DNA fragment of the ITS2 rRNA gene region of each sample was individually amplified and subjected to high-throughput NGS sequencing. Analysis of 168,933 high-quality reads showed the presence of 690 fungal taxa. Investigation of microbial ASVs diversity revealed plant-dependent and postharvest handling-affected fungal assemblages. Among the microorganisms inhabiting tested berries, potentially beneficial or pathogenic fungi were documented. Numerous cultivable yeasts were isolated from the surface of tested berries and characterized by their antagonistic activity. Some of the isolates, identified as Aureobasidium pullulans, Metschnikowia fructicola, and M. pulcherrima, displayed pronounced activity against potential fungal pathogens and showed attractiveness for disease control. MDPI 2021-06-30 /pmc/articles/PMC8307871/ /pubmed/34209423 http://dx.doi.org/10.3390/microorganisms9071423 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Stanevičienė, Ramunė
Lukša, Juliana
Strazdaitė-Žielienė, Živilė
Ravoitytė, Bazilė
Losinska-Sičiūnienė, Regina
Mozūraitis, Raimondas
Servienė, Elena
Mycobiota in the Carposphere of Sour and Sweet Cherries and Antagonistic Features of Potential Biocontrol Yeasts
title Mycobiota in the Carposphere of Sour and Sweet Cherries and Antagonistic Features of Potential Biocontrol Yeasts
title_full Mycobiota in the Carposphere of Sour and Sweet Cherries and Antagonistic Features of Potential Biocontrol Yeasts
title_fullStr Mycobiota in the Carposphere of Sour and Sweet Cherries and Antagonistic Features of Potential Biocontrol Yeasts
title_full_unstemmed Mycobiota in the Carposphere of Sour and Sweet Cherries and Antagonistic Features of Potential Biocontrol Yeasts
title_short Mycobiota in the Carposphere of Sour and Sweet Cherries and Antagonistic Features of Potential Biocontrol Yeasts
title_sort mycobiota in the carposphere of sour and sweet cherries and antagonistic features of potential biocontrol yeasts
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8307871/
https://www.ncbi.nlm.nih.gov/pubmed/34209423
http://dx.doi.org/10.3390/microorganisms9071423
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