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Mycobiota in the Carposphere of Sour and Sweet Cherries and Antagonistic Features of Potential Biocontrol Yeasts
Sour cherries (Prunus cerasus L.) and sweet cherries (P. avium L.) are economically important fruits with high potential in the food industry and medicine. In this study, we analyzed fungal communities associated with the carposphere of sour and sweet cherries that were freshly harvested from privat...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8307871/ https://www.ncbi.nlm.nih.gov/pubmed/34209423 http://dx.doi.org/10.3390/microorganisms9071423 |
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author | Stanevičienė, Ramunė Lukša, Juliana Strazdaitė-Žielienė, Živilė Ravoitytė, Bazilė Losinska-Sičiūnienė, Regina Mozūraitis, Raimondas Servienė, Elena |
author_facet | Stanevičienė, Ramunė Lukša, Juliana Strazdaitė-Žielienė, Živilė Ravoitytė, Bazilė Losinska-Sičiūnienė, Regina Mozūraitis, Raimondas Servienė, Elena |
author_sort | Stanevičienė, Ramunė |
collection | PubMed |
description | Sour cherries (Prunus cerasus L.) and sweet cherries (P. avium L.) are economically important fruits with high potential in the food industry and medicine. In this study, we analyzed fungal communities associated with the carposphere of sour and sweet cherries that were freshly harvested from private plantations and purchased in a food store. Following DNA isolation, a DNA fragment of the ITS2 rRNA gene region of each sample was individually amplified and subjected to high-throughput NGS sequencing. Analysis of 168,933 high-quality reads showed the presence of 690 fungal taxa. Investigation of microbial ASVs diversity revealed plant-dependent and postharvest handling-affected fungal assemblages. Among the microorganisms inhabiting tested berries, potentially beneficial or pathogenic fungi were documented. Numerous cultivable yeasts were isolated from the surface of tested berries and characterized by their antagonistic activity. Some of the isolates, identified as Aureobasidium pullulans, Metschnikowia fructicola, and M. pulcherrima, displayed pronounced activity against potential fungal pathogens and showed attractiveness for disease control. |
format | Online Article Text |
id | pubmed-8307871 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-83078712021-07-25 Mycobiota in the Carposphere of Sour and Sweet Cherries and Antagonistic Features of Potential Biocontrol Yeasts Stanevičienė, Ramunė Lukša, Juliana Strazdaitė-Žielienė, Živilė Ravoitytė, Bazilė Losinska-Sičiūnienė, Regina Mozūraitis, Raimondas Servienė, Elena Microorganisms Article Sour cherries (Prunus cerasus L.) and sweet cherries (P. avium L.) are economically important fruits with high potential in the food industry and medicine. In this study, we analyzed fungal communities associated with the carposphere of sour and sweet cherries that were freshly harvested from private plantations and purchased in a food store. Following DNA isolation, a DNA fragment of the ITS2 rRNA gene region of each sample was individually amplified and subjected to high-throughput NGS sequencing. Analysis of 168,933 high-quality reads showed the presence of 690 fungal taxa. Investigation of microbial ASVs diversity revealed plant-dependent and postharvest handling-affected fungal assemblages. Among the microorganisms inhabiting tested berries, potentially beneficial or pathogenic fungi were documented. Numerous cultivable yeasts were isolated from the surface of tested berries and characterized by their antagonistic activity. Some of the isolates, identified as Aureobasidium pullulans, Metschnikowia fructicola, and M. pulcherrima, displayed pronounced activity against potential fungal pathogens and showed attractiveness for disease control. MDPI 2021-06-30 /pmc/articles/PMC8307871/ /pubmed/34209423 http://dx.doi.org/10.3390/microorganisms9071423 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Stanevičienė, Ramunė Lukša, Juliana Strazdaitė-Žielienė, Živilė Ravoitytė, Bazilė Losinska-Sičiūnienė, Regina Mozūraitis, Raimondas Servienė, Elena Mycobiota in the Carposphere of Sour and Sweet Cherries and Antagonistic Features of Potential Biocontrol Yeasts |
title | Mycobiota in the Carposphere of Sour and Sweet Cherries and Antagonistic Features of Potential Biocontrol Yeasts |
title_full | Mycobiota in the Carposphere of Sour and Sweet Cherries and Antagonistic Features of Potential Biocontrol Yeasts |
title_fullStr | Mycobiota in the Carposphere of Sour and Sweet Cherries and Antagonistic Features of Potential Biocontrol Yeasts |
title_full_unstemmed | Mycobiota in the Carposphere of Sour and Sweet Cherries and Antagonistic Features of Potential Biocontrol Yeasts |
title_short | Mycobiota in the Carposphere of Sour and Sweet Cherries and Antagonistic Features of Potential Biocontrol Yeasts |
title_sort | mycobiota in the carposphere of sour and sweet cherries and antagonistic features of potential biocontrol yeasts |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8307871/ https://www.ncbi.nlm.nih.gov/pubmed/34209423 http://dx.doi.org/10.3390/microorganisms9071423 |
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