Calcein Binding to Assess Mineralization in Hydrogel Microspheres

We describe a method to assess mineralization by osteoblasts within microspheres using calcein. Fluorescence imaging of calcein bound to the calcium in hydroxyapatite permits assessment of the mineralized portion of the extracellular matrix. Colorimetric imaging of Alizarin Red S complexed with calc...

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Detalles Bibliográficos
Autores principales: White, Kristopher, Chalaby, Rabab, Lowe, Gina, Berlin, Jacob, Glackin, Carlotta, Olabisi, Ronke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8309385/
https://www.ncbi.nlm.nih.gov/pubmed/34301032
http://dx.doi.org/10.3390/polym13142274

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8309385/
https://www.ncbi.nlm.nih.gov/pubmed/34301032
http://dx.doi.org/10.3390/polym13142274

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