Validation and implementation of a direct RT-qPCR method for rapid screening of SARS-CoV-2 infection by using non-invasive saliva samples

OBJECTIVE: To validate and implement an optimized screening method for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA combining use of self-collected raw saliva samples, single-step heat-treated virus inactivation and RNA extraction, and direct RT-qPCR. METHODS: Th...

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Detalles Bibliográficos
Autores principales: Brotons, Pedro, Perez-Argüello, Amaresh, Launes, Cristian, Torrents, Francesc, Subirats, Maria Pilar, Saucedo, Jesica, Claverol, Joana, Garcia-Garcia, Juan Jose, Rodas, Gil, Fumado, Vicky, Jordan, Iolanda, Gratacos, Eduard, Bassat, Quique, Muñoz-Almagro, Carmen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Authors. Published by Elsevier Ltd on behalf of International Society for Infectious Diseases. 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8310572/
https://www.ncbi.nlm.nih.gov/pubmed/34320412
http://dx.doi.org/10.1016/j.ijid.2021.07.054

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8310572/
https://www.ncbi.nlm.nih.gov/pubmed/34320412
http://dx.doi.org/10.1016/j.ijid.2021.07.054

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