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Development of a dual-labeled, hydrolysis probe-based, real-time quantitative PCR assay for detection of both genotypes of duck circovirus-1 (DuCV-1) and DuCV-2

In this study, we developed a real-time quantitative polymerase chain reaction (qPCR) assay based on a dual-labeled hydrolysis probe to simultaneously detect both duck circovirus (DuCV) 1 and DuCV-2. The reproducibility, sensitivity and specificity of the primer set and probe were evaluated using ot...

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Detalles Bibliográficos
Autores principales: Zhang, Lin, Jiang, Wenming, Zhang, Fuyou, Li, Yang, Li, Jinping, Liang, Shaobo, Yu, Xiaohui, Peng, Cheng, Liu, Shuo, Wang, Jingjing, Sun, Shuhong, Liu, Hualei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8312713/
https://www.ncbi.nlm.nih.gov/pubmed/34312741
http://dx.doi.org/10.1007/s11262-021-01862-9