Development of a dual-labeled, hydrolysis probe-based, real-time quantitative PCR assay for detection of both genotypes of duck circovirus-1 (DuCV-1) and DuCV-2
In this study, we developed a real-time quantitative polymerase chain reaction (qPCR) assay based on a dual-labeled hydrolysis probe to simultaneously detect both duck circovirus (DuCV) 1 and DuCV-2. The reproducibility, sensitivity and specificity of the primer set and probe were evaluated using ot...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer US
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8312713/ https://www.ncbi.nlm.nih.gov/pubmed/34312741 http://dx.doi.org/10.1007/s11262-021-01862-9 |
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author | Zhang, Lin Jiang, Wenming Zhang, Fuyou Li, Yang Li, Jinping Liang, Shaobo Yu, Xiaohui Peng, Cheng Liu, Shuo Wang, Jingjing Sun, Shuhong Liu, Hualei |
author_facet | Zhang, Lin Jiang, Wenming Zhang, Fuyou Li, Yang Li, Jinping Liang, Shaobo Yu, Xiaohui Peng, Cheng Liu, Shuo Wang, Jingjing Sun, Shuhong Liu, Hualei |
author_sort | Zhang, Lin |
collection | PubMed |
description | In this study, we developed a real-time quantitative polymerase chain reaction (qPCR) assay based on a dual-labeled hydrolysis probe to simultaneously detect both duck circovirus (DuCV) 1 and DuCV-2. The reproducibility, sensitivity and specificity of the primer set and probe were evaluated using other duck pathogens. The detection limit was 20 copies per µL. The intra-assay coefficients of variation (CVs) were ≤ 0.73% and the inter-assay CVs were ≤ 1.89%. No cross-reaction occurred with other duck pathogens. In addition, the qPCR assay was successfully applied to the simultaneous detection of DuCV-1 and DuCV-2 in clinical field samples. Therefore, this assay will be useful for laboratory diagnosis and epidemiological field studies of DuCV. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s11262-021-01862-9. |
format | Online Article Text |
id | pubmed-8312713 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Springer US |
record_format | MEDLINE/PubMed |
spelling | pubmed-83127132021-07-26 Development of a dual-labeled, hydrolysis probe-based, real-time quantitative PCR assay for detection of both genotypes of duck circovirus-1 (DuCV-1) and DuCV-2 Zhang, Lin Jiang, Wenming Zhang, Fuyou Li, Yang Li, Jinping Liang, Shaobo Yu, Xiaohui Peng, Cheng Liu, Shuo Wang, Jingjing Sun, Shuhong Liu, Hualei Virus Genes Short Report In this study, we developed a real-time quantitative polymerase chain reaction (qPCR) assay based on a dual-labeled hydrolysis probe to simultaneously detect both duck circovirus (DuCV) 1 and DuCV-2. The reproducibility, sensitivity and specificity of the primer set and probe were evaluated using other duck pathogens. The detection limit was 20 copies per µL. The intra-assay coefficients of variation (CVs) were ≤ 0.73% and the inter-assay CVs were ≤ 1.89%. No cross-reaction occurred with other duck pathogens. In addition, the qPCR assay was successfully applied to the simultaneous detection of DuCV-1 and DuCV-2 in clinical field samples. Therefore, this assay will be useful for laboratory diagnosis and epidemiological field studies of DuCV. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s11262-021-01862-9. Springer US 2021-07-26 2021 /pmc/articles/PMC8312713/ /pubmed/34312741 http://dx.doi.org/10.1007/s11262-021-01862-9 Text en © The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2021 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. |
spellingShingle | Short Report Zhang, Lin Jiang, Wenming Zhang, Fuyou Li, Yang Li, Jinping Liang, Shaobo Yu, Xiaohui Peng, Cheng Liu, Shuo Wang, Jingjing Sun, Shuhong Liu, Hualei Development of a dual-labeled, hydrolysis probe-based, real-time quantitative PCR assay for detection of both genotypes of duck circovirus-1 (DuCV-1) and DuCV-2 |
title | Development of a dual-labeled, hydrolysis probe-based, real-time quantitative PCR assay for detection of both genotypes of duck circovirus-1 (DuCV-1) and DuCV-2 |
title_full | Development of a dual-labeled, hydrolysis probe-based, real-time quantitative PCR assay for detection of both genotypes of duck circovirus-1 (DuCV-1) and DuCV-2 |
title_fullStr | Development of a dual-labeled, hydrolysis probe-based, real-time quantitative PCR assay for detection of both genotypes of duck circovirus-1 (DuCV-1) and DuCV-2 |
title_full_unstemmed | Development of a dual-labeled, hydrolysis probe-based, real-time quantitative PCR assay for detection of both genotypes of duck circovirus-1 (DuCV-1) and DuCV-2 |
title_short | Development of a dual-labeled, hydrolysis probe-based, real-time quantitative PCR assay for detection of both genotypes of duck circovirus-1 (DuCV-1) and DuCV-2 |
title_sort | development of a dual-labeled, hydrolysis probe-based, real-time quantitative pcr assay for detection of both genotypes of duck circovirus-1 (ducv-1) and ducv-2 |
topic | Short Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8312713/ https://www.ncbi.nlm.nih.gov/pubmed/34312741 http://dx.doi.org/10.1007/s11262-021-01862-9 |
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