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A piggyBac‐mediated transgenesis system for the temporary expression of CRISPR/Cas9 in rice

Targeted mutagenesis via CRISPR/Cas9 is now widely used, not only in model plants but also in agriculturally important crops. However, in vegetative crop propagation, CRISPR/Cas9 expression cassettes cannot be segregated out in the resulting progenies, but must nevertheless be eliminated without lea...

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Detalles Bibliográficos
Autores principales: Nishizawa‐Yokoi, Ayako, Toki, Seiichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8313132/
https://www.ncbi.nlm.nih.gov/pubmed/33529430
http://dx.doi.org/10.1111/pbi.13559