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Reverse transcription priming methods affect normalisation choices for gene expression levels in oocytes and early embryos

Mammalian oocytes and embryos rely exclusively on maternal mRNAs to accomplish early developmental processes. Since oocytes and early embryos are transcriptionally silent after meiotic resumption, most of the synthesised maternal mRNA does not undergo immediate translation but is instead stored in t...

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Detalles Bibliográficos
Autores principales: Yu, Bo, van Tol, Helena T A, Stout, Tom A E, Roelen, Bernard A J
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8314208/
https://www.ncbi.nlm.nih.gov/pubmed/34152407
http://dx.doi.org/10.1093/molehr/gaab040