RNA diversification by a self-reproducing ribozyme revealed by deep sequencing and kinetic modelling

We demonstrate that a recombinase ribozyme achieves multiple functions in the same reaction network: self-reproduction, iterative elongation and circularization of other RNAs, leading to synthesis of diverse products predicted by a kinetic model. This shows that key mechanisms can be integrated and...

Descripción completa

Detalles Bibliográficos
Autores principales: Jeancolas, Cyrille, Matsubara, Yoshiya J., Vybornyi, Mykhailo, Lambert, Camille N., Blokhuis, Alex, Alline, Thomas, Griffiths, Andrew D., Ameta, Sandeep, Krishna, Sandeep, Nghe, Philippe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2021
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8320737/
https://www.ncbi.nlm.nih.gov/pubmed/34235521
http://dx.doi.org/10.1039/d1cc02290c
_version_ 1783730696745910272
author Jeancolas, Cyrille
Matsubara, Yoshiya J.
Vybornyi, Mykhailo
Lambert, Camille N.
Blokhuis, Alex
Alline, Thomas
Griffiths, Andrew D.
Ameta, Sandeep
Krishna, Sandeep
Nghe, Philippe
author_facet Jeancolas, Cyrille
Matsubara, Yoshiya J.
Vybornyi, Mykhailo
Lambert, Camille N.
Blokhuis, Alex
Alline, Thomas
Griffiths, Andrew D.
Ameta, Sandeep
Krishna, Sandeep
Nghe, Philippe
author_sort Jeancolas, Cyrille
collection PubMed
description We demonstrate that a recombinase ribozyme achieves multiple functions in the same reaction network: self-reproduction, iterative elongation and circularization of other RNAs, leading to synthesis of diverse products predicted by a kinetic model. This shows that key mechanisms can be integrated and controlled toward Darwinian evolution in RNA reaction networks.
format Online
Article
Text
id pubmed-8320737
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher The Royal Society of Chemistry
record_format MEDLINE/PubMed
spelling pubmed-83207372021-08-09 RNA diversification by a self-reproducing ribozyme revealed by deep sequencing and kinetic modelling Jeancolas, Cyrille Matsubara, Yoshiya J. Vybornyi, Mykhailo Lambert, Camille N. Blokhuis, Alex Alline, Thomas Griffiths, Andrew D. Ameta, Sandeep Krishna, Sandeep Nghe, Philippe Chem Commun (Camb) Chemistry We demonstrate that a recombinase ribozyme achieves multiple functions in the same reaction network: self-reproduction, iterative elongation and circularization of other RNAs, leading to synthesis of diverse products predicted by a kinetic model. This shows that key mechanisms can be integrated and controlled toward Darwinian evolution in RNA reaction networks. The Royal Society of Chemistry 2021-07-06 /pmc/articles/PMC8320737/ /pubmed/34235521 http://dx.doi.org/10.1039/d1cc02290c Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Jeancolas, Cyrille
Matsubara, Yoshiya J.
Vybornyi, Mykhailo
Lambert, Camille N.
Blokhuis, Alex
Alline, Thomas
Griffiths, Andrew D.
Ameta, Sandeep
Krishna, Sandeep
Nghe, Philippe
RNA diversification by a self-reproducing ribozyme revealed by deep sequencing and kinetic modelling
title RNA diversification by a self-reproducing ribozyme revealed by deep sequencing and kinetic modelling
title_full RNA diversification by a self-reproducing ribozyme revealed by deep sequencing and kinetic modelling
title_fullStr RNA diversification by a self-reproducing ribozyme revealed by deep sequencing and kinetic modelling
title_full_unstemmed RNA diversification by a self-reproducing ribozyme revealed by deep sequencing and kinetic modelling
title_short RNA diversification by a self-reproducing ribozyme revealed by deep sequencing and kinetic modelling
title_sort rna diversification by a self-reproducing ribozyme revealed by deep sequencing and kinetic modelling
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8320737/
https://www.ncbi.nlm.nih.gov/pubmed/34235521
http://dx.doi.org/10.1039/d1cc02290c
work_keys_str_mv AT jeancolascyrille rnadiversificationbyaselfreproducingribozymerevealedbydeepsequencingandkineticmodelling
AT matsubarayoshiyaj rnadiversificationbyaselfreproducingribozymerevealedbydeepsequencingandkineticmodelling
AT vybornyimykhailo rnadiversificationbyaselfreproducingribozymerevealedbydeepsequencingandkineticmodelling
AT lambertcamillen rnadiversificationbyaselfreproducingribozymerevealedbydeepsequencingandkineticmodelling
AT blokhuisalex rnadiversificationbyaselfreproducingribozymerevealedbydeepsequencingandkineticmodelling
AT allinethomas rnadiversificationbyaselfreproducingribozymerevealedbydeepsequencingandkineticmodelling
AT griffithsandrewd rnadiversificationbyaselfreproducingribozymerevealedbydeepsequencingandkineticmodelling
AT ametasandeep rnadiversificationbyaselfreproducingribozymerevealedbydeepsequencingandkineticmodelling
AT krishnasandeep rnadiversificationbyaselfreproducingribozymerevealedbydeepsequencingandkineticmodelling
AT nghephilippe rnadiversificationbyaselfreproducingribozymerevealedbydeepsequencingandkineticmodelling

Ejemplares similares