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CBFB-MYH11 Fusion Sequesters RUNX1 in Cytoplasm to Prevent DNMT3A Recruitment to Target Genes in AML

A growing number of human diseases have been found to be associated with aberrant DNA methylation, including cancer. Mutations targeting genes encoding DNA methyltransferase (DNMT), TET family of DNA demethylases, and isocitrate dehydrogenase (IDH1, IDH2) that produce TET inhibitory metabolite, 2-hy...

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Autores principales: Liu, Peng, Liu, Jin-Pin, Sun, Si-Jia, Gao, Yun, Ai, Yingjie, Chen, Xiufei, Sun, Yiping, Zhou, Mengyu, Liu, Yun, Xiong, Yue, Yuan, Hai-Xin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8321512/
https://www.ncbi.nlm.nih.gov/pubmed/34336831
http://dx.doi.org/10.3389/fcell.2021.675424
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author Liu, Peng
Liu, Jin-Pin
Sun, Si-Jia
Gao, Yun
Ai, Yingjie
Chen, Xiufei
Sun, Yiping
Zhou, Mengyu
Liu, Yun
Xiong, Yue
Yuan, Hai-Xin
author_facet Liu, Peng
Liu, Jin-Pin
Sun, Si-Jia
Gao, Yun
Ai, Yingjie
Chen, Xiufei
Sun, Yiping
Zhou, Mengyu
Liu, Yun
Xiong, Yue
Yuan, Hai-Xin
author_sort Liu, Peng
collection PubMed
description A growing number of human diseases have been found to be associated with aberrant DNA methylation, including cancer. Mutations targeting genes encoding DNA methyltransferase (DNMT), TET family of DNA demethylases, and isocitrate dehydrogenase (IDH1, IDH2) that produce TET inhibitory metabolite, 2-hyoxyglutarate (2-HG), are found in more than half of acute myeloid leukemia (AML). To gain new insights into the regulation of DNA de/methylation and consequence of its alteration in cancer development, we searched for genes which are mutated in a manner that is linked with gene mutations involved in DNA de/methylation in multiple cancer types. We found that recurrent CBFB-MYH11 fusions, which result in the expression of fusion protein comprising core-binding factor β (CBFB) and myosin heavy chain 11 (MYH11) and are found in 6∼8% of AML patients, occur mutually exclusively with DNMT3A mutations. Tumors bearing CBFB-MYH11 fusion show DNA hypomethylation patterns similar to those with loss-of-function mutation of DNMT3A. Expression of CBFB-MYH11 fusion or inhibition of DNMT3A similarly impairs the methylation and expression of target genes of Runt related transcription factor 1 (RUNX1), a functional partner of CBFB. We demonstrate that RUNX1 directly interacts with DNMT3A and that CBFB-MYH11 fusion protein sequesters RUNX1 in the cytoplasm, thereby preventing RUNX1 from interacting with and recruiting DNMT3A to its target genes. Our results identify a novel regulation of DNA methylation and provide a molecular basis how CBFB-MYH11 fusion contributes to leukemogenesis.
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spelling pubmed-83215122021-07-30 CBFB-MYH11 Fusion Sequesters RUNX1 in Cytoplasm to Prevent DNMT3A Recruitment to Target Genes in AML Liu, Peng Liu, Jin-Pin Sun, Si-Jia Gao, Yun Ai, Yingjie Chen, Xiufei Sun, Yiping Zhou, Mengyu Liu, Yun Xiong, Yue Yuan, Hai-Xin Front Cell Dev Biol Cell and Developmental Biology A growing number of human diseases have been found to be associated with aberrant DNA methylation, including cancer. Mutations targeting genes encoding DNA methyltransferase (DNMT), TET family of DNA demethylases, and isocitrate dehydrogenase (IDH1, IDH2) that produce TET inhibitory metabolite, 2-hyoxyglutarate (2-HG), are found in more than half of acute myeloid leukemia (AML). To gain new insights into the regulation of DNA de/methylation and consequence of its alteration in cancer development, we searched for genes which are mutated in a manner that is linked with gene mutations involved in DNA de/methylation in multiple cancer types. We found that recurrent CBFB-MYH11 fusions, which result in the expression of fusion protein comprising core-binding factor β (CBFB) and myosin heavy chain 11 (MYH11) and are found in 6∼8% of AML patients, occur mutually exclusively with DNMT3A mutations. Tumors bearing CBFB-MYH11 fusion show DNA hypomethylation patterns similar to those with loss-of-function mutation of DNMT3A. Expression of CBFB-MYH11 fusion or inhibition of DNMT3A similarly impairs the methylation and expression of target genes of Runt related transcription factor 1 (RUNX1), a functional partner of CBFB. We demonstrate that RUNX1 directly interacts with DNMT3A and that CBFB-MYH11 fusion protein sequesters RUNX1 in the cytoplasm, thereby preventing RUNX1 from interacting with and recruiting DNMT3A to its target genes. Our results identify a novel regulation of DNA methylation and provide a molecular basis how CBFB-MYH11 fusion contributes to leukemogenesis. Frontiers Media S.A. 2021-07-15 /pmc/articles/PMC8321512/ /pubmed/34336831 http://dx.doi.org/10.3389/fcell.2021.675424 Text en Copyright © 2021 Liu, Liu, Sun, Gao, Ai, Chen, Sun, Zhou, Liu, Xiong and Yuan. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Liu, Peng
Liu, Jin-Pin
Sun, Si-Jia
Gao, Yun
Ai, Yingjie
Chen, Xiufei
Sun, Yiping
Zhou, Mengyu
Liu, Yun
Xiong, Yue
Yuan, Hai-Xin
CBFB-MYH11 Fusion Sequesters RUNX1 in Cytoplasm to Prevent DNMT3A Recruitment to Target Genes in AML
title CBFB-MYH11 Fusion Sequesters RUNX1 in Cytoplasm to Prevent DNMT3A Recruitment to Target Genes in AML
title_full CBFB-MYH11 Fusion Sequesters RUNX1 in Cytoplasm to Prevent DNMT3A Recruitment to Target Genes in AML
title_fullStr CBFB-MYH11 Fusion Sequesters RUNX1 in Cytoplasm to Prevent DNMT3A Recruitment to Target Genes in AML
title_full_unstemmed CBFB-MYH11 Fusion Sequesters RUNX1 in Cytoplasm to Prevent DNMT3A Recruitment to Target Genes in AML
title_short CBFB-MYH11 Fusion Sequesters RUNX1 in Cytoplasm to Prevent DNMT3A Recruitment to Target Genes in AML
title_sort cbfb-myh11 fusion sequesters runx1 in cytoplasm to prevent dnmt3a recruitment to target genes in aml
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8321512/
https://www.ncbi.nlm.nih.gov/pubmed/34336831
http://dx.doi.org/10.3389/fcell.2021.675424
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