Differentiation of human pluripotent stem cells into functional airway basal stem cells

Airway basal cells play an essential role in the maintenance of the airway epithelium. Here, we provide a detailed directed differentiation protocol to generate ‘‘induced basal cells (iBCs)’’ from human pluripotent stem cells. iBCs recapitulate biological and functional properties of airway basal ce...

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Detalles Bibliográficos
Autores principales: Suzuki, Shingo, Hawkins, Finn J., Barillà, Cristina, Beermann, Mary Lou, Kotton, Darrell N., Davis, Brian R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8322461/
https://www.ncbi.nlm.nih.gov/pubmed/34355203
http://dx.doi.org/10.1016/j.xpro.2021.100683
Descripción
Sumario:Airway basal cells play an essential role in the maintenance of the airway epithelium. Here, we provide a detailed directed differentiation protocol to generate ‘‘induced basal cells (iBCs)’’ from human pluripotent stem cells. iBCs recapitulate biological and functional properties of airway basal cells including mucociliary differentiation in vitro or in vivo in tracheal xenografts, facilitating the study of inherited and acquired diseases of the airway, as well as potential use in regenerative medicine. For complete details on the use and execution of this protocol, please refer to Hawkins et al. (2021).

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