Differentiation of human pluripotent stem cells into functional airway basal stem cells

Airway basal cells play an essential role in the maintenance of the airway epithelium. Here, we provide a detailed directed differentiation protocol to generate ‘‘induced basal cells (iBCs)’’ from human pluripotent stem cells. iBCs recapitulate biological and functional properties of airway basal ce...

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Detalles Bibliográficos
Autores principales: Suzuki, Shingo, Hawkins, Finn J., Barillà, Cristina, Beermann, Mary Lou, Kotton, Darrell N., Davis, Brian R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8322461/
https://www.ncbi.nlm.nih.gov/pubmed/34355203
http://dx.doi.org/10.1016/j.xpro.2021.100683
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author Suzuki, Shingo
Hawkins, Finn J.
Barillà, Cristina
Beermann, Mary Lou
Kotton, Darrell N.
Davis, Brian R.
author_facet Suzuki, Shingo
Hawkins, Finn J.
Barillà, Cristina
Beermann, Mary Lou
Kotton, Darrell N.
Davis, Brian R.
author_sort Suzuki, Shingo
collection PubMed
description Airway basal cells play an essential role in the maintenance of the airway epithelium. Here, we provide a detailed directed differentiation protocol to generate ‘‘induced basal cells (iBCs)’’ from human pluripotent stem cells. iBCs recapitulate biological and functional properties of airway basal cells including mucociliary differentiation in vitro or in vivo in tracheal xenografts, facilitating the study of inherited and acquired diseases of the airway, as well as potential use in regenerative medicine. For complete details on the use and execution of this protocol, please refer to Hawkins et al. (2021).
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spelling pubmed-83224612021-08-04 Differentiation of human pluripotent stem cells into functional airway basal stem cells Suzuki, Shingo Hawkins, Finn J. Barillà, Cristina Beermann, Mary Lou Kotton, Darrell N. Davis, Brian R. STAR Protoc Protocol Airway basal cells play an essential role in the maintenance of the airway epithelium. Here, we provide a detailed directed differentiation protocol to generate ‘‘induced basal cells (iBCs)’’ from human pluripotent stem cells. iBCs recapitulate biological and functional properties of airway basal cells including mucociliary differentiation in vitro or in vivo in tracheal xenografts, facilitating the study of inherited and acquired diseases of the airway, as well as potential use in regenerative medicine. For complete details on the use and execution of this protocol, please refer to Hawkins et al. (2021). Elsevier 2021-07-20 /pmc/articles/PMC8322461/ /pubmed/34355203 http://dx.doi.org/10.1016/j.xpro.2021.100683 Text en © 2021 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Suzuki, Shingo
Hawkins, Finn J.
Barillà, Cristina
Beermann, Mary Lou
Kotton, Darrell N.
Davis, Brian R.
Differentiation of human pluripotent stem cells into functional airway basal stem cells
title Differentiation of human pluripotent stem cells into functional airway basal stem cells
title_full Differentiation of human pluripotent stem cells into functional airway basal stem cells
title_fullStr Differentiation of human pluripotent stem cells into functional airway basal stem cells
title_full_unstemmed Differentiation of human pluripotent stem cells into functional airway basal stem cells
title_short Differentiation of human pluripotent stem cells into functional airway basal stem cells
title_sort differentiation of human pluripotent stem cells into functional airway basal stem cells
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8322461/
https://www.ncbi.nlm.nih.gov/pubmed/34355203
http://dx.doi.org/10.1016/j.xpro.2021.100683
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